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. 1982 Oct;70(4):994–998. doi: 10.1104/pp.70.4.994

Sulfite-Induced Lipid Peroxidation in Chloroplasts as Determined by Ethane Production 1

Galen D Peiser 1, Ma Concepcion C Lizada 1,2, Shang Fa Yang 1
PMCID: PMC1065813  PMID: 16662657

Abstract

Ethane formation, as a measure of lipid peroxidation, was studied in spinach (Spinacia oleracea L.) chloroplasts exposed to sulfite. Ethane formation required sulfite and light, and occurred with concomitant oxidation of sulfite to sulfate. In the dark, both ethane formation and sulfite oxidation were inhibited. Ethane formation was stimulated by ferric or ferrous ions and inhibited by ethylenediamine tetraacetate. The photosynthetic electron transport modulators, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and phenazine methosulfate, inhibited both sulfite oxidation and ethane formation. Methyl viologen greatly stimulated ethane formation, but had little effect on sulfite oxidation. Methyl viologen, in the absence of sulfite, caused only a small amount of ethane formation in comparison to that produced with sulfite alone. Sulfite oxidation and ethane formation were effectively inhibited by the radical scavengers, 1,2-dihydroxybenzene-3,5-disulfonic acid and ascorbate. Ethanol, a hydroxyl radical scavenger, inhibited ethane formation only to a small degree; formate, which converts hydroxyl radical to superoxide radical, caused a small stimulation in both sulfite oxidation and ethane formation. Superoxide dismutase inhibited ethane formation by 50% when added at a concentration equivalent to that of the endogenous activity. Singlet oxygen did not appear to play a role in ethane formation, inasmuch as the singlet oxygen scavengers, sodium azide and 1,4-diazobicyclo-[2,2,2]-octane, were not inhibitory. These data are consistent with the view that O2 is reduced by the photosynthetic electron transport system to superoxide anion, which in turn initiates the free radical oxidation of sulfite, and the free radicals produced during sulfite oxidation were responsible for the peroxidation of membrane lipids, resulting in the formation of ethane.

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Selected References

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  1. Asada K., Kiso K. Initiation of aerobic oxidation of sulfite by illuminated spinach chloroplasts. Eur J Biochem. 1973 Mar 1;33(2):253–257. doi: 10.1111/j.1432-1033.1973.tb02677.x. [DOI] [PubMed] [Google Scholar]
  2. Bors W., Saran M., Michel C. Pulse-radiolytic investigations of catechols and catecholamines. II. Reactions of Tiron with oxygen radical species. Biochim Biophys Acta. 1979 Feb 1;582(3):537–542. doi: 10.1016/0304-4165(79)90145-4. [DOI] [PubMed] [Google Scholar]
  3. Dillard C. J., Tappel A. L. Volatile hydrocarbon and carbonyl products of lipid peroxidation: a comparison of pentane, ethane, hexanal, and acetone as in vivo indices. Lipids. 1979 Dec;14(12):989–995. doi: 10.1007/BF02533435. [DOI] [PubMed] [Google Scholar]
  4. Dumelin E. E., Tappel A. L. Hydrocarbon gases produced during in vitro peroxidation of polyunsaturated fatty acids and decomposition of preformed hydroperoxides. Lipids. 1977 Nov;12(11):894–900. doi: 10.1007/BF02533308. [DOI] [PubMed] [Google Scholar]
  5. FRIDOVICH I., HANDLER P. Detection of free radicals generated during enzymic oxidations by the initiation of sulfite oxidation. J Biol Chem. 1961 Jun;236:1836–1840. [PubMed] [Google Scholar]
  6. Golbeck J. H., Lien S., San Pietro A. Isolation and characterization of a subchloroplast particle enriched in iron-sulfur protein and P700. Arch Biochem Biophys. 1977 Jan 15;178(1):140–150. doi: 10.1016/0003-9861(77)90178-3. [DOI] [PubMed] [Google Scholar]
  7. Greenstock C. L., Miller R. W. The oxidation of tiron by superoxide anion. Kinetics of the reaction in aqueous solution in chloroplasts. Biochim Biophys Acta. 1975 Jul 8;396(1):11–16. doi: 10.1016/0005-2728(75)90184-x. [DOI] [PubMed] [Google Scholar]
  8. Halliwell B. The biological effects of the superoxide radical and its products. Bull Eur Physiopathol Respir. 1981;17 (Suppl):21–29. [PubMed] [Google Scholar]
  9. Kaplan D., McJilton C., Luchtel D. Bisulfite induced lipid oxidation. Arch Environ Health. 1975 Oct;30(10):507–509. doi: 10.1080/00039896.1975.10666764. [DOI] [PubMed] [Google Scholar]
  10. Kochi J. K. Mechanisms of organic oxidation and reduction by metal complexes. Science. 1967 Jan 27;155(3761):415–424. doi: 10.1126/science.155.3761.415. [DOI] [PubMed] [Google Scholar]
  11. Kong S., Davison A. J. The role of interactions between O2, H2O2, .OH,e- and O2- in free radical damage to biological systems. Arch Biochem Biophys. 1980 Oct 1;204(1):18–29. doi: 10.1016/0003-9861(80)90003-x. [DOI] [PubMed] [Google Scholar]
  12. McCord J. M., Day E. D., Jr Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex. FEBS Lett. 1978 Feb 1;86(1):139–142. doi: 10.1016/0014-5793(78)80116-1. [DOI] [PubMed] [Google Scholar]
  13. Packer J. E., Mahood J. S., Mora-Arellano V. O., Slater T. F., Willson R. L., Wolfenden B. S. Free radicals and singlet oxygen scavengers: reaction of a peroxy-radical with beta-carotene, diphenyl furan and 1,4-diazobicyclo (2,2,2)-octane. Biochem Biophys Res Commun. 1981 Feb 27;98(4):901–906. doi: 10.1016/0006-291x(81)91196-7. [DOI] [PubMed] [Google Scholar]
  14. Peiser G. D., Yang S. F. Chlorophyll destruction by the bisulfite-oxygen system. Plant Physiol. 1977 Aug;60(2):277–281. doi: 10.1104/pp.60.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riely C. A., Cohen G., Lieberman M. Ethane evolution: a new index of lipid peroxidation. Science. 1974 Jan 18;183(4121):208–210. doi: 10.1126/science.183.4121.208. [DOI] [PubMed] [Google Scholar]
  16. Scaringelli F. P., Saltzman B. E., Frey S. A. Spectrophotometric determination of atmospheric sulfur dioxide. Anal Chem. 1967 Dec;39(14):1709–1726. doi: 10.1021/ac50157a031. [DOI] [PubMed] [Google Scholar]
  17. Tuazon P. T., Johnson S. L. Free radical and ionic reaction of bisulfite with reduced nicotinamide adenine dinucleotide and its analogues. Biochemistry. 1977 Mar 22;16(6):1183–1188. doi: 10.1021/bi00625a024. [DOI] [PubMed] [Google Scholar]
  18. Yang S. F. Destruction of tryptophan during the aerobic oxidation of sulfite ions. Environ Res. 1973 Dec;6(4):395–402. doi: 10.1016/0013-9351(73)90055-8. [DOI] [PubMed] [Google Scholar]
  19. Yang S. F. Sulfoxide formation from methionine or its sulfide analogs during aerobic oxidation of sulfite. Biochemistry. 1970 Dec 8;9(25):5008–5014. doi: 10.1021/bi00827a027. [DOI] [PubMed] [Google Scholar]

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