Abstract
Nitrate or ammonium was added to soybean (Glycine max L. Merrill cv Corsoy) plants grown in plastic pouches 10 days after nodules first appeared. By the third day of treatment with 10 millimolar nitrate, nitrogenase specific activity (per unit nodule weight) had decreased to 15% to 25% of that of untreated plants. Longer incubations and higher concentrations of nitrate had no greater effect. In addition, exogenous nitrate or ammonium resulted in slower nodule growth and decreased total protein synthesis in both the bacterial and the plant portion of the nodule (as measured by incorporation of 35S). Two-dimensional gel electrophoresis revealed that the nitrogenase components were not repressed or degraded relative to other bacteroid proteins. In the presence of an optimal carbon source, the nitrogenase specific activity of nodules detached from nitrate-treated plants was equivalent to that of nodules from untreated plants. These results are consistent with models that propose decreased availability or utilization of photosynthate in root nodules when legumes are exposed to fixed nitrogen.
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- Bisseling T., van den Bos R. C., van Kammen A. The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum. Biochim Biophys Acta. 1978 Feb 13;539(1):1–11. doi: 10.1016/0304-4165(78)90115-0. [DOI] [PubMed] [Google Scholar]
- Chen P. C., Phillips D. A. Induction of Root Nodule Senescence by Combined Nitrogen in Pisum sativum L. Plant Physiol. 1977 Mar;59(3):440–442. doi: 10.1104/pp.59.3.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy R. W., Holsten R. D., Jackson E. K., Burns R. C. The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43(8):1185–1207. doi: 10.1104/pp.43.8.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helleiner C. W., Wunner W. H. A simple method for counting 14C- and 3H-proteins in polyacrylamide gels. Anal Biochem. 1971 Feb;39(2):333–338. doi: 10.1016/0003-2697(71)90423-4. [DOI] [PubMed] [Google Scholar]
- Houwaard F. Effect of Ammonium Chloride and Methionine Sulfoximine on the Acetylene Reduction of Detached Root Nodules of Peas (Pisum sativum). Appl Environ Microbiol. 1979 Jan;37(1):73–79. doi: 10.1128/aem.37.1.73-79.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Houwaard F. Influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (Pisum sativum). Appl Environ Microbiol. 1978 Jun;35(6):1061–1065. doi: 10.1128/aem.35.6.1061-1065.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maier R. J., Brill W. J. Ineffective and non-nodulating mutant strains of Rhizobium japonicum. J Bacteriol. 1976 Aug;127(2):763–769. doi: 10.1128/jb.127.2.763-769.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Rigaud J., Puppo A. Effect of nitrite upon leghemoglobin and interaction with nitrogen fixation. Biochim Biophys Acta. 1977 May 26;497(3):702–706. doi: 10.1016/0304-4165(77)90291-4. [DOI] [PubMed] [Google Scholar]
- Roberts G. P., Brill W. J. Genetics and regulation of nitrogen fixation. Annu Rev Microbiol. 1981;35:207–235. doi: 10.1146/annurev.mi.35.100181.001231. [DOI] [PubMed] [Google Scholar]
- Sutton W. D., Jepsen N. M. Studies with detached lupin root nodules in culture: I. Maintenance and induction of acetylene reduction activity. Plant Physiol. 1975 Nov;56(5):665–670. doi: 10.1104/pp.56.5.665. [DOI] [PMC free article] [PubMed] [Google Scholar]