Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Nov;70(5):1380–1384. doi: 10.1104/pp.70.5.1380

Preparation of Avocado Mitochondria Using Self-Generated Percoll Density Gradients and Changes in Buoyant Density during Ripening

Francois Moreau 1,1, Roger Romani 1,2
PMCID: PMC1065891  PMID: 16662683

Abstract

Mitochondria from avocado (Persea americana Mill, var. Fuerte and Hass) can be rapidly prepared at every stage of ripening using differential centrifugation and self-generated Percoll gradients. The procedure results in improved oxidative and phosphorylative properties, especially for mitochondria isolated from preclimacteric fruits.

A gradual change in the buoyant density of avocado mitochondria takes place during ripening. Climacteric and postclimacteric avocado mitochondria have the same buoyant density as other plant mitochondria (potato, cauliflower), whereas mitochondria from preclimacteric fruit have a lower density. The transition in buoyant density occurs during the climacteric rise, and two populations of intact mitochondria (p = 1.060 and p = 1.075) can be separated at this stage. Evidence indicates that the difference in mitochondrial buoyant density between preclimacteric and postclimacteric mitochondria is likely due to interactions with soluble cytosolic components.

Full text

PDF
1380

Images in this article

1381Fig. 1
on p.1381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergman A., Gardeström P., Ericson I. Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach Leaves. Plant Physiol. 1980 Sep;66(3):442–445. doi: 10.1104/pp.66.3.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goldstein A. H., Anderson J. O., McDaniel R. G. Cyanide-insensitive and Cyanide-sensitive O(2) Uptake in Wheat: I. GRADIENT-PURIFIED MITOCHONDRIA. Plant Physiol. 1980 Sep;66(3):488–493. doi: 10.1104/pp.66.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jackson C., Dench J. E., Hall D. O., Moore A. L. Separation of mitochondria from contaminating subcellular structures utilizing silica sol gradient centrifugation. Plant Physiol. 1979 Jul;64(1):150–153. doi: 10.1104/pp.64.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LUCK D. F. FORMATION OF MITOCHONDRIA IN NEUROSPORA CRASSA. A STUDY BASED ON MITOCHONDRIAL DENSITY CHANGES. J Cell Biol. 1965 Mar;24:461–470. doi: 10.1083/jcb.24.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lance C., Hobson G. E., Young R. E., Biale J. B. Metabolic processes in cytoplasmic particles of the avocado fruit. VII. Oxidative and phosphorylative activities throughout the climacteric cycle. Plant Physiol. 1965 Nov;40(6):1116–1123. doi: 10.1104/pp.40.6.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lilley R. M. Isolation of Functionally Intact Rhodoplasts from Griffithsia monilis (Ceramiaceae, Rhodophyta). Plant Physiol. 1981 Jan;67(1):5–8. doi: 10.1104/pp.67.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moreau F., Romani R. Malate Oxidation and Cyanide-Insensitive Respiration in Avocado Mitochondria during the Climacteric Cycle. Plant Physiol. 1982 Nov;70(5):1385–1390. doi: 10.1104/pp.70.5.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ozelkök S. I., Romani R. J. Ripening and in vitro retention of respiratory control by avocado and pear mitochondria. Plant Physiol. 1975 Aug;56(2):239–244. doi: 10.1104/pp.56.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tolbert N. E. Isolation of subcellular organelles of metabolism on isopycnic sucrose gradients. Methods Enzymol. 1974;31:734–746. doi: 10.1016/0076-6879(74)31077-4. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES