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. 1982 Nov;70(5):1405–1409. doi: 10.1104/pp.70.5.1405

An Assessment of Phase Transitions in Soybean Membranes

Sharman D O'Neill 1,1, A Carl Leopold 1
PMCID: PMC1065896  PMID: 16662688

Abstract

Phase transitions were measured in vesicles of phospholipids, alone and in various combinations, and in pelleted mitochondrial membranes, using thermal (DSC) and optical methods. The objective was to consider their possible involvement in chilling injury of soybeans (Glycine max [L.] Merr. cv Wayne 1977). Saturated phospholipids showed clear transitions in the temperature range of 50°C to near 0°C. When mixtures of two phospholipids were examined, there was a marked lowering and broadening of the transition peaks, and a shift in the transition temperatures to intermediate temperatures. The unsaturated phospholipids that occur naturally in soybeans showed no detectable phase transitions in this temperature range, alone or in combinations. Examination of the polar lipids from soybean asolectin revealed no transitions in the biological temperature range; the additions of cations such as Ca2+ and La3+ did not evoke a detectable phase transition in them. Mitochondrial membrane pellets likewise showed no transitions. The application of these two direct methods of examination of membrane components without the addition of foreign agents did not support the suggested occurrence of a bulk phase transition which could be related to chilling injury in soybeans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Lee A. G. Lipid phase transitions and phase diagrams. I. Lipid phase transitions. Biochim Biophys Acta. 1977 Aug 9;472(2):237–281. doi: 10.1016/0304-4157(77)90018-1. [DOI] [PubMed] [Google Scholar]
  3. Lee A. G. Lipid phase transitions and phase diagrams. II. Mictures involving lipids. Biochim Biophys Acta. 1977 Nov 14;472(3-4):285–344. doi: 10.1016/0304-4157(77)90001-6. [DOI] [PubMed] [Google Scholar]
  4. Leopold A. C., Musgrave M. E. Respiratory changes with chilling injury of soybeans. Plant Physiol. 1979 Nov;64(5):702–705. doi: 10.1104/pp.64.5.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lyons J. M., Raison J. K. Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. Plant Physiol. 1970 Apr;45(4):386–389. doi: 10.1104/pp.45.4.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mabrey S., Sturtevant J. M. Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862–3866. doi: 10.1073/pnas.73.11.3862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Petersen N. O., Kroon P. A., Kainoshio M., Chan S. I. Thermal phase transitions in deuterated lecithin bilayers. Chem Phys Lipids. 1975 Aug;14(4):343–349. doi: 10.1016/0009-3084(75)90071-7. [DOI] [PubMed] [Google Scholar]
  8. Philipson K. D., Bers D. M., Nishimoto A. Y. The role of phospholipids in the Ca2+ binding of isolated cardiac sarcolemma. J Mol Cell Cardiol. 1980 Nov;12(11):1159–1173. doi: 10.1016/0022-2828(80)90063-2. [DOI] [PubMed] [Google Scholar]
  9. Priestley D. A., Leopold A. C. Absence of Lipid Oxidation during Accelerated Aging of Soybean Seeds. Plant Physiol. 1979 Apr;63(4):726–729. doi: 10.1104/pp.63.4.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Raison J. K. Temperature-induced phase changes in membrane lipids and their influence on metabolic regulation. Symp Soc Exp Biol. 1973;27:485–512. [PubMed] [Google Scholar]
  11. Scherer G. F., Morré D. J. Action and Inhibition of Endogenous Phospholipases during Isolation of Plant Membranes. Plant Physiol. 1978 Dec;62(6):933–937. doi: 10.1104/pp.62.6.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  13. Sturtevant J. M., Ho C., Reimann A. Thermotropic behavior of some fluorodimyristoylphosphatidylcholines. Proc Natl Acad Sci U S A. 1979 May;76(5):2239–2243. doi: 10.1073/pnas.76.5.2239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Szoka F., Jr, Papahadjopoulos D. Comparative properties and methods of preparation of lipid vesicles (liposomes). Annu Rev Biophys Bioeng. 1980;9:467–508. doi: 10.1146/annurev.bb.09.060180.002343. [DOI] [PubMed] [Google Scholar]
  15. Wilson R. F., Rinne R. W. Phospholipids in the developing soybean seed. Plant Physiol. 1974 Nov;54(5):744–747. doi: 10.1104/pp.54.5.744. [DOI] [PMC free article] [PubMed] [Google Scholar]

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