Abstract
The synthesis of α-tocopherol from 2,3-dimethylphytylquinol and S-adenosyl-l-methionine was achieved using Capsicum annuum fruit chromoplasts. The enzymes involved in the cyclization (2,3-dimethyl-phytylquinol cyclase) and methylation (S-adenosyl methionine:γ-tocopherol methyl-transferase) are both localized in the chromoplast membrane fraction (envelopes and/or a-chlorophyll lamellae), in contrast to the stroma fraction.
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Selected References
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- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Nakatani H. Y., Barber J. An improved method for isolating chloroplasts retaining their outer membranes. Biochim Biophys Acta. 1977 Sep 14;461(3):500–512. [PubMed] [Google Scholar]
- Soll J., Kemmerling M., Schultz G. Tocopherol and plastoquinone synthesis in spinach chloroplasts subfractions. Arch Biochem Biophys. 1980 Oct 15;204(2):544–550. doi: 10.1016/0003-9861(80)90066-1. [DOI] [PubMed] [Google Scholar]
- Williams J. P. Separation and estimation of quinones and alpha-tocopherol from Vicia faba leaves. J Chromatogr. 1968 Sep 10;36(4):504–511. doi: 10.1016/s0021-9673(01)92978-4. [DOI] [PubMed] [Google Scholar]