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. 1982 Dec;70(6):1664–1666. doi: 10.1104/pp.70.6.1664

Biosynthesis of Indol-3-yl-acetyl-myo-inositol Arabinoside in Kernels of Zea mays L. 1

Luis J Corcuera 1,2, Robert S Bandurski 1
PMCID: PMC1065951  PMID: 16662740

Abstract

Extracts of immature kernels of Zea mays L. catalyzed the synthesis of indol-3-yl-acetyl-myo-inositol arabinoside from indol-3-yl-acetyl-myo-inositol and UDP-[U-14C]xylose. The product contained radioactivity which upon hydrolysis with trifluoroacetic acid cochromatographed with arabinose and not xylose. The amount of product from the reaction was proportional to the amount of indol-3-yl-acetyl-myo-inositol added, and the product was positive to Ehmann's reagent for indoles. In addition, the product and authentic indol-3-yl-acetyl-myo-inositol arabinoside had the same RF or retention time in three chromatographic systems.

By analogy to the wheat germ system, it is proposed that a UDP-d-xylose 4-epimerase is present in immature kernels of maize and that this enzyme catalyzes the conversion of UDP-d-xylose to UDP-l-arabinose, the probable sugar donor to indol-3-yl-acetyl-myo-inositol.

The present data demonstrates the presence of a previously undescribed enzyme, UDP-arabinose:indol-3-yl-acetyl-myo-inositol arabinosyl transferase in maize kernels. This work together with our prior studies provides a pathway for the biosynthesis of all the low molecular weight esters of indol-3-yl-acetic acid in maize kernels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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