Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1982 Dec;70(6):1667–1672. doi: 10.1104/pp.70.6.1667

Spectral Evidence for a Component Involved in Hydrogen Metabolism of Soybean Nodule Bacteroids 1

Günter Eisbrenner 1,2, Harold J Evans 1
PMCID: PMC1065952  PMID: 16662741

Abstract

A component with a difference spectrum similar to that of b-type cytochromes which becomes reduced upon the addition of H2 has been demonstrated in soybean nodule bacteroids. This electron carrier, referred to as component 559-H2, is present in hydrogenase-positive strains of Rhizobium japonicum but has not been detected in mutants that lack hydrogenase activity or in hydrogenase-negative wild-type strains. A positive correlation between concentrations of component 559-H2 and hydrogenase activities has been established. These results provide further evidence that component 559-H2 is involved in H2 metabolism in R. japonicum.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams M. W., Mortenson L. E., Chen J. S. Hydrogenase. Biochim Biophys Acta. 1980 Dec;594(2-3):105–176. doi: 10.1016/0304-4173(80)90007-5. [DOI] [PubMed] [Google Scholar]
  2. Appleby C. A. Electron transport systems of Rhizobium japonicum. I. Haemoprotein P-450, other CO-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochim Biophys Acta. 1969 Jan 14;172(1):71–87. doi: 10.1016/0005-2728(69)90093-0. [DOI] [PubMed] [Google Scholar]
  3. Appleby C. A. Electron transport systems of Rhizobium japonicum. II. Rhizobium haemoglobin, cytochromes and oxidases in free-living (cultured) cells. Biochim Biophys Acta. 1969 Jan 14;172(1):88–105. doi: 10.1016/0005-2728(69)90094-2. [DOI] [PubMed] [Google Scholar]
  4. Arp D. J., Burris R. H. Purification and properties of the particulate hydrogenase from the bacteroids of soybean root nodules. Biochim Biophys Acta. 1979 Oct 11;570(2):221–230. doi: 10.1016/0005-2744(79)90142-6. [DOI] [PubMed] [Google Scholar]
  5. Carter K. R., Jennings N. T., Hanus J., Evans H. J. Hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of Rhizobium japonicum. Can J Microbiol. 1978 Mar;24(3):307–311. doi: 10.1139/m78-051. [DOI] [PubMed] [Google Scholar]
  6. Eisbrenner G., Evans H. J. Carriers in electron transport from molecular hydrogen to oxygen in Rhizobium japonicum bacteroids. J Bacteriol. 1982 Mar;149(3):1005–1012. doi: 10.1128/jb.149.3.1005-1012.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lepo J. E., Hanus F. J., Evans H. J. Chemoautotrophic growth of hydrogen-uptake-positive strains of Rhizobium japonicum. J Bacteriol. 1980 Feb;141(2):664–670. doi: 10.1128/jb.141.2.664-670.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lepo J. E., Hickok R. E., Cantrell M. A., Russell S. A., Evans H. J. Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum. J Bacteriol. 1981 May;146(2):614–620. doi: 10.1128/jb.146.2.614-620.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maier R. J., Campbell N. E., Hanus F. J., Simpson F. B., Russell S. A., Evans H. J. Expression of hydrogenase activity in free-living Rhizobium japonicum. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3258–3262. doi: 10.1073/pnas.75.7.3258. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES