Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1982 Dec;70(6):1767–1769. doi: 10.1104/pp.70.6.1767

Synthesis of Oat Globulin Precursors 1

Analogy to Legume 11S Storage Protein Synthesisa

A Chris Brinegar 1,2, David M Peterson 1,2
PMCID: PMC1065971  PMID: 16662760

Abstract

The oat (Avena sativa L.) seed globulin was found to be synthesized in vitro as 60,000 to 64,000 dalton precursors. In vivo protein labeling yielded polypeptides of 58,000 to 62,000 daltons, suggesting cleavage of signal sequences from the precursors. Further cleavage is apparently required to separate the α and β polypeptide sequences which are known to form disulfide-linked 53,000 to 58,000 dalton species in the (αβ)6 holoprotein. The data are discussed with respect to analogous synthesis and processing of some legume 11S storage proteins.

Full text

PDF
1767

Images in this article

1768Fig. 1
on p.1768
1768Fig. 2
on p.1768

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bantle J. A., Maxwell I. H., Hahn W. E. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976 May 7;72:413–427. doi: 10.1016/0003-2697(76)90549-2. [DOI] [PubMed] [Google Scholar]
  2. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. doi: 10.1083/jcb.67.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burr B., Burr F. A., St John T. P., Thomas M., Davis R. W. Zein storage protein gene family of maize. An assessment of heterogeneity with cloned messenger RNA sequences. J Mol Biol. 1982 Jan 5;154(1):33–49. doi: 10.1016/0022-2836(82)90415-6. [DOI] [PubMed] [Google Scholar]
  4. Burr F. A., Burr B. In vitro uptake and processing of prezein and other maize preproteins by maize membranes. J Cell Biol. 1981 Aug;90(2):427–434. doi: 10.1083/jcb.90.2.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ereken-Tumer N., Richter J. D., Nielsen N. C. Structural characterization of the glycinin precursors. J Biol Chem. 1982 Apr 25;257(8):4016–4018. [PubMed] [Google Scholar]
  6. Greene F. C. In Vitro Synthesis of Wheat (Triticum aestivum L.) Storage Proteins. Plant Physiol. 1981 Sep;68(3):778–783. doi: 10.1104/pp.68.3.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kim S. I., Charbonnier L., Mossé J. Heterogeneity of avenin, the oat prolamin. Fractionation, molecular weight and amino acid composition. Biochim Biophys Acta. 1978 Nov 20;537(1):22–30. doi: 10.1016/0005-2795(78)90599-8. [DOI] [PubMed] [Google Scholar]
  8. Konzak C. F. Genetic control of the content, amino acid composition, and processing properties of proteins in wheat. Adv Genet. 1977;19:407–582. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Larkins B. A., Hurkman W. J. Synthesis and deposition of zein in protein bodies of maize endosperm. Plant Physiol. 1978 Aug;62(2):256–263. doi: 10.1104/pp.62.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lizardi P. M., Engelberg A. Rapid isolation of RNA using proteinase K and sodium perchlorate. Anal Biochem. 1979 Sep 15;98(1):116–122. doi: 10.1016/0003-2697(79)90714-0. [DOI] [PubMed] [Google Scholar]
  12. Luthe D. S., Peterson D. M. Cell-free Synthesis of Globulin by Developing Oat (Avena sativa L.) Seeds. Plant Physiol. 1977 May;59(5):836–841. doi: 10.1104/pp.59.5.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Okita T. W., Greene F. C. Wheat Storage Proteins : ISOLATION AND CHARACTERIZATION OF THE GLIADIN MESSENGER RNAs. Plant Physiol. 1982 Apr;69(4):834–839. doi: 10.1104/pp.69.4.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Peterson D. M. Subunit structure and composition of oat seed globulin. Plant Physiol. 1978 Oct;62(4):506–509. doi: 10.1104/pp.62.4.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riezman H., Weir E. M., Leaver C. J., Titus D. E., Becker W. M. Regulation of Glyoxysomal Enzymes during Germination of Cucumber: 3. IN VITRO TRANSLATION AND CHARACTERIZATION OF FOUR GLYOXYSOMAL ENZYMES. Plant Physiol. 1980 Jan;65(1):40–46. doi: 10.1104/pp.65.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tumer N. E., Thanh V. H., Nielsen N. C. Purification and characterization of mRNA from soybean seeds. Identification of glycinin and beta-conglycinin precursors. J Biol Chem. 1981 Aug 25;256(16):8756–8760. [PubMed] [Google Scholar]
  17. Weeke B. Rocket immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:37–46. doi: 10.1111/j.1365-3083.1973.tb03777.x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES