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. 1983 Feb;71(2):404–408. doi: 10.1104/pp.71.2.404

Relationship between the Kinetic Properties and the Small Subunit Composition of Nicotiana Ribulose-1, 5-bisphosphate Carboxylase 1

L R Li 1,2, Verne A Sisson 1,2,2, S D Kung 1,2
PMCID: PMC1066045  PMID: 16662838

Abstract

Genetic variability in the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) in several Nicotiana species has been characterized by isoelectric focusing patterns. This heritable variation provides an opportunity to examine the functional role of each of these subunits. In this study, specifically designed RuBPCase enzymes composed of identical large subunits but different small subunits were constructed in vivo by interspecific hybridization between the species N. sylvestris, N. tabacum, N. glauca, N. glutinosa, N. plumbaginifolia, and N. tomentosiformis. Small subunit polypeptides were combined to form a sequence of one, two, three, and four polypeptides with the large subunit of N. sylvestris. Kinetic properties of these hybrid enzymes were compared. No differences in the specific activity of either carboxylation or oxygenation nor in Km values for ribulose 1,5-bisphosphate, CO2, or O2 were detected among the RuBPCase enzymes from the various interspecific hybrids. Likewise, the ratio of carboxylation to oxygenation was constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews T. J., Abel K. M. Kinetics and subunit interactions of ribulose bisphosphate carboxylase-oxygenase from the cyanobacterium, Synechococcus sp. J Biol Chem. 1981 Aug 25;256(16):8445–8451. [PubMed] [Google Scholar]
  2. Chan P. H., Sakano K., Singh S., Wildman S. G. Crystalline fraction I protein: preparation in large yield. Science. 1972 Jun 9;176(4039):1145–1146. doi: 10.1126/science.176.4039.1145. [DOI] [PubMed] [Google Scholar]
  3. Hatch A. L., Jensen R. G. Regulation of ribulose-1,5-bisphosphate carboxylase from tobacco: changes in pH response and affinity for CO2 and Mg2+ induced by chloroplast intermediates. Arch Biochem Biophys. 1980 Dec;205(2):587–594. doi: 10.1016/0003-9861(80)90142-3. [DOI] [PubMed] [Google Scholar]
  4. Hirai A. Random assembly of different kinds of small subunit polypeptides during formation of fraction I protein macromolecules. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3443–3445. doi: 10.1073/pnas.74.8.3443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kung S. D., Sakano K., Wildman S. G. Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electrofocusing. Biochim Biophys Acta. 1974 Sep 13;365(1):138–147. doi: 10.1016/0005-2795(74)90258-x. [DOI] [PubMed] [Google Scholar]
  6. Kung S. Tobacco fraction 1 protein: a unique genetic marker. Science. 1976 Feb 6;191(4226):429–434. doi: 10.1126/science.1108201. [DOI] [PubMed] [Google Scholar]
  7. Nishimura M., Akazawa T. Reconstitution of spinach ribulose-1,5-diphosphate carboxylase from departed subunits. Biochem Biophys Res Commun. 1974 Jul 24;59(2):584–590. doi: 10.1016/s0006-291x(74)80020-3. [DOI] [PubMed] [Google Scholar]
  8. Oliver D. J., Zelitch I. Increasing photosynthesis by inhibiting photorespiration with glyoxylate. Science. 1977 Jun 24;196(4297):1450–1451. doi: 10.1126/science.867040. [DOI] [PubMed] [Google Scholar]
  9. Rhodes P. R., Kung S. D., Marsho T. V. Relationship of Ribulose-1,5-bisphosphate Carboxylase-Oxygenase Specific Activity to Subunit Composition. Plant Physiol. 1980 Jan;65(1):69–73. doi: 10.1104/pp.65.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tabita F. R., McFadden B. A. D-ribulose 1,5-diphosphate carboxylase from Rhodospirillum rubrum. II. Quaternary structure, composition, catalytic, and immunological properties. J Biol Chem. 1974 Jun 10;249(11):3459–3464. [PubMed] [Google Scholar]
  11. Zelitch I. Improving the efficiency of photosynthesis. Science. 1975 May 9;188(4188):626–633. doi: 10.1126/science.188.4188.626. [DOI] [PubMed] [Google Scholar]

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