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. 1983 Apr;71(4):912–915. doi: 10.1104/pp.71.4.912

Inhibition of Glutamine Synthetase from Pea by Tabtoxinine-β-lactam

Michael D Thomas 1,2, Pat J Langston-Unkefer 1,2, Thomas F Uchytil 1,2, Richard D Durbin 1,2
PMCID: PMC1066143  PMID: 16662928

Abstract

Tabtoxinine-β-lactam, a hydrolytic product of tabtoxin produced by Pseudomonas syringae pv. tabaci, apparently inactivates pea seed glutamine synthetase. Inhibition of the enzyme's initial velocity is linear over a range of 0.5 to 5 millimolar tabtoxinine-β-lactam in the presence of 10 millimolar glutamate. A method for the purification of glutamine synthetase from dried peas is presented which gives a 30% yield with a 2,000-fold increase in specific activity. A method for obtaining highly purified tabtoxinine-β-lactam and tabtoxin in good yields is also presented. The authenticity and purity of tabtoxinine-β-lactam and tabtoxin were verified by chromatography, biological activity, and 1H and 13C nuclear magnetic resonance spectroscopy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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