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. 1983 Jul;72(3):858–863. doi: 10.1104/pp.72.3.858

Mass Spectrometric Analysis of Cytokinins in Plant Tissues 1

V. Identification of the Cytokinin Complex of Datura Innoxia Crown Gall Tissue

Lok Man S Palni 1,2, Roger E Summons 1, David S Letham 1
PMCID: PMC1066334  PMID: 16663099

Abstract

The cytokinin complex of Datura innoxia Mill. crown gall tissue was purified by ion exchange, Sephadex LH-20 chromatography and reversed-phase high performance liquid chromatography. By gas chromatography-mass spectrometry using 2H-labeled compounds, the following cytokinins were identified in the basic fraction eluting from a cation exchange column: zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, their corresponding O-glucosides, 7- and 9-glucosides of zeatin, 9-glucoside of dihydrozeatin, isopentenyladenine, and isopentenyladenosine. Zeatin riboside 5′-monophosphate was the major cytokinin nucleotide in the tissue. In addition, dihydrozeatin riboside and isopentenyladenosine were identified in the nucleotide fraction following enymic degradation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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