Abstract
The total glycine pool in Zea mays L. Mo17×B73 leaf discs was measured after steady state photosynthesis in 50%, 21% and 1% O2. The glycine pool was a function of O2 concentration; it was largest in 50% O2 and smallest in 1% O2. Incubation of discs with methyl hydroxybutynoic acid in 21% O2 in the light caused an accumulation of carbon in glycolate. This accumulation was O2 sensitive, as subsequent photosynthetic periods in 50%, 21%, and 1% O2 resulted in the largest glycolate pool in 50% O2 and the smallest in 1% O2. At the same time, the O2-dependent increase in the glycine pool was eliminated. After untreated leaf discs reached steady state photosynthesis in 21% O2, measurements made subsequently in darkness, or in 1% O2 in the light, showed that the glycine pool decreased. On the basis of these results, we conclude that a major portion of the total glycine pool in corn is an intermediate in the photorespiratory glycolate pathway. Considering both the rate of decay of the glycine pool in the dark and the rate of decay of the glycine pool after changing from 21% to 1% O2, we conclude that this glycine pool is turning over slowly.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowes G., Ogren W. L. Oxygen inhibition and other properties of soybean ribulose 1,5-diphosphate carboxylase. J Biol Chem. 1972 Apr 10;247(7):2171–2176. [PubMed] [Google Scholar]
- Créach E., Stewart C. R. Effects of aminoacetonitrile on net photosynthesis, ribulose-1,5-bisphosphate levels, and glycolate pathway intermediates. Plant Physiol. 1982 Nov;70(5):1444–1448. doi: 10.1104/pp.70.5.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hitz W. D., Stewart C. R. Oxygen and Carbon Dioxide Effects on the Pool Size of Some Photosynthetic and Photorespiratory Intermediates in Soybean (Glycine max [L.] Merr.). Plant Physiol. 1980 Mar;65(3):442–446. doi: 10.1104/pp.65.3.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jewess P. J., Kerr M. W., Whitaker D. P. Inhibition of glycollate oxidase from pea leaves. FEBS Lett. 1975 May 15;53(3):292–296. doi: 10.1016/0014-5793(75)80039-1. [DOI] [PubMed] [Google Scholar]
- Morot-Gaudry J. F. Oxygen effect on photosynthetic and glycolate pathways in young maize leaves. Plant Physiol. 1980 Dec;66(6):1079–1084. doi: 10.1104/pp.66.6.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osmond C. B., Harris B. Photorespiration during C 4 photosynthesis. Biochim Biophys Acta. 1971 May 11;234(2):270–282. doi: 10.1016/0005-2728(71)90082-x. [DOI] [PubMed] [Google Scholar]