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. Author manuscript; available in PMC: 2024 Aug 15.
Published in final edited form as: Biochemistry. 2023 Jul 25;62(16):2461–2471. doi: 10.1021/acs.biochem.3c00180

Table 5.

Steady-state kinetic parameters for hhCF-4OT, fCF-4OT, and hhCF-4OT variants using 2-HM.

Enzyme kcat (s−1) Km (μM) kcat/Km (M−1 s−1)

hhCF-4OT 30 ± 1 80 ± 10 3.8 × 105
fCF-4OT 180 ± 10 290 ± 25 6.2 × 105
CFαP1A 0.40 ± 0.04 120 ± 20 3.3 × 103
CFαL11Y 80 ± 4 190 ± 20 4.2 × 105
CFαR39A 15 ± 5 750 ± 210 2.0 × 104
CFβR11A 0.07 ± 0.004 140 ± 20 5.0 × 102
CFβR39A 40 ± 3 230 ± 25 1.7 × 105
CFβR62A 9 ± 1 400 ± 60 2.3 × 104