Abstract
An apparatus is described which permits the simultaneous measurement of O2 evolution and chlorophyll a fluorescence from illuminated discs or pieces of green leaves. O2 is measured in the gas phase in a temperature-controlled chamber of approximately 5-milliliter capacity. Calibration is effected by injection of air through vents. Response time is approximately 1.5 seconds for O2, and full scale deflection, in normal operating mode, is approximately 10 micromoles O2. The apparatus may also be used to monitor fluorescence alone, in an open mode, in which gas is passed continuously through the chamber.
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- Walker D. A., Sivak M. N., Prinsley R. T., Cheesbrough J. K. Simultaneous measurement of oscillations in oxygen evolution and chlorophyll a fluorescence in leaf pieces. Plant Physiol. 1983 Nov;73(3):542–549. doi: 10.1104/pp.73.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]