Abstract
A 6-hour application (6-hour pulse) of 1 millimolar azide significantly changed the phase of the potassium uptake rhythm of Lemna gibba G3. The phase response curve obtained was type 0 and very similar to that caused by a 6-hour pulse of low temperature (5°C) or darkness. The magnitude of the phase shift and the type of the phase response curve depended on the concentration of azide. However, 6-hour pulses of 3 millimolar cyanide or 10 micromolar (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to shift the phase of the rhythm, while these pulses lowered the rate of carbon dioxide uptake or release. Azide, even at 3 micromolar, selectively reduced the amplitude of the rhythm without inhibiting the mean level of potassium uptake.
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