Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1983 Nov;73(3):636–638. doi: 10.1104/pp.73.3.636

Microsomal Membrane Changes during the Ripening of Apple Fruit 1

Susan Lurie 1, Ruth Ben-Arie 1
PMCID: PMC1066520  PMID: 16663272

Abstract

The changes in leakage and viscosity of microsomal membranes from apples (Malus sylvestris cv Calville de San Sauveur) at different stages of ripening were examined. These changes were correlated with those in the lipid composition of the membranes, sterols, phospholipids, and fatty acids of the phospholipids. The greatest changes in membrane properties occurred as the fruit reached its climacteric and this corresponded with a change in the sterol:phospholipid ratio in the membranes. Changes were also found in fatty acid unsaturation level, but primarily in the postclimacteric stage of ripening.

Full text

PDF
636

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutelmann P., Kende H. Membrane Lipids in Senescing Flower Tissue of Ipomoea tricolor. Plant Physiol. 1977 May;59(5):888–893. doi: 10.1104/pp.59.5.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borochov A., Halevy A. H. Microviscosity of plasmalemmas in rose petals as affected by age and environmental factors. Plant Physiol. 1978 May;61(5):812–815. doi: 10.1104/pp.61.5.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. CHIAMORI N., HENRY R. J. Study of the ferric chloride method for determination of total cholesterol and cholesterol esters. Am J Clin Pathol. 1959 Apr;31(4):305–309. doi: 10.1093/ajcp/31.4.305. [DOI] [PubMed] [Google Scholar]
  5. Eletr S., Zakim D., Vessey D. A. A spin-label study of the role of phospholipids in the regulation of membrane-bound microsomal enzymes. J Mol Biol. 1973 Aug 5;78(2):351–362. doi: 10.1016/0022-2836(73)90121-6. [DOI] [PubMed] [Google Scholar]
  6. Esfahani M., Limbrick A. R., Knutton S., Oka T., Wakil S. J. The molecular organization of lipids in the membrane of Escherichia coli: phase transitions. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3180–3184. doi: 10.1073/pnas.68.12.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McKersie B. D., Thompson J. E. Lipid crystallization in senescent membranes from cotyledons. Plant Physiol. 1977 May;59(5):803–807. doi: 10.1104/pp.59.5.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McKersie B. D., Thompson J. E. Phase properties of senescing plant membranes: role of the neutral lipids. Biochim Biophys Acta. 1979 Jan 5;550(1):48–58. doi: 10.1016/0005-2736(79)90114-7. [DOI] [PubMed] [Google Scholar]
  9. RENKONEN O., KOSUNEN T. U., RENKONEN O. V. EXTRACTION OF SERUM INOSITIDES AND OTHER PHOSPHATIDES. Ann Med Exp Biol Fenn. 1963;41:375–381. [PubMed] [Google Scholar]
  10. Sacher J. A. Studies of permeability, RNA and protein turnover during ageing of fruit and leaf tissues. Symp Soc Exp Biol. 1967;21:269–303. [PubMed] [Google Scholar]
  11. Shechter E., Letellier L., Gulik-Krzywicki G. Relations between structure and function in cytoplasmic membrane vesicles isolated from an Escherichia coli fatty-acid auxotroph. High-angle x-ray diffraction, freeze-etch electron microscopy and transport studies. Eur J Biochem. 1974 Nov 1;49(1):61–76. doi: 10.1111/j.1432-1033.1974.tb03811.x. [DOI] [PubMed] [Google Scholar]
  12. Shinitzky M., Barenholz Y. Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta. 1978 Dec 15;515(4):367–394. doi: 10.1016/0304-4157(78)90010-2. [DOI] [PubMed] [Google Scholar]
  13. Suttle J. C., Kende H. Ethylene Action and Loss of Membrane Integrity during Petal Senescence in Tradescantia. Plant Physiol. 1980 Jun;65(6):1067–1072. doi: 10.1104/pp.65.6.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES