Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1983 Nov;73(3):698–702. doi: 10.1104/pp.73.3.698

Release of Calcium from Suspension-Cultured Glycine max Cells by Chitosan, Other Polycations, and Polyamines in Relation to Effects on Membrane Permeability 1

David H Young 1,2, Heinrich Kauss 1
PMCID: PMC1066533  PMID: 16663285

Abstract

Treatment with chitosan of suspension-cultured Glycine max cells labeled with 45Ca2+ caused a rapid release of calcium, which was complete much earlier than the chitosan-induced leakage of intracellular electrolytes and probably reflects calcium loss primarily from the cell wall and/or plasma membrane. A linear correlation was found between calcium release from chitosan-treated whole cells or isolated cell walls and the amount of chitosan bound. Other polycations (poly-l-lysine, histone, DEAE-dextran, and protamine sulfate), low molecular weight polyamines (spermine, spermidine, and putrescine) and polyanions (polygalacturonate and poly-l-aspartate, which act as chelating agents) also released calcium from whole cells and isolated cell walls; however, only the polycations increased membrane permeability. Poly-l-lysines of differing molecular weight showed a similar ability to release calcium, but their effect on membrane permeability increased with increasing molecular weight. The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components. The order of effectiveness of inorganic cations in displacing calcium from whole cells and isolated cell walls was Ca2+, Ba2+, Sr2+ > Mg2+ > K+, Na+.

Full text

PDF
698

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ebel J., Schaller-Hekeler B., Knobloch K. H., Wellman E., Grisebach H., Hahlbrock K. Coordinated changes in enzyme activities of phenylpropanoid metabolism during the growth of soybean cell suspension cultures. Biochim Biophys Acta. 1974 Oct 8;362(3):417–424. doi: 10.1016/0304-4165(74)90137-8. [DOI] [PubMed] [Google Scholar]
  2. English P. D., Jurale J. B., Albersheim P. Host-Pathogen Interactions: II. Parameters Affecting Polysaccharide-degrading Enzyme Secretion by Colletotrichum lindemuthianum Grown in Culture. Plant Physiol. 1971 Jan;47(1):1–6. doi: 10.1104/pp.47.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fuhrer J., Kaur-Sawhney R., Shih L. M., Galston A. W. Effects of Exogenous 1,3-Diaminopropane and Spermidine on Senescence of Oat Leaves : II. Inhibition of Ethylene Biosynthesis and Possible Mode of Action. Plant Physiol. 1982 Dec;70(6):1597–1600. doi: 10.1104/pp.70.6.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  5. Hadwiger L. A., Beckman J. M., Adams M. J. Localization of Fungal Components in the Pea-Fusarium Interaction Detected Immunochemically with Anti-chitosan and Anti-fungal Cell Wall Antisera. Plant Physiol. 1981 Jan;67(1):170–175. doi: 10.1104/pp.67.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hadwiger L. A., Beckman J. M. Chitosan as a Component of Pea-Fusarium solani Interactions. Plant Physiol. 1980 Aug;66(2):205–211. doi: 10.1104/pp.66.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ruesink A. W. The plasma membrane of Avena coleoptile protoplasts. Plant Physiol. 1971 Feb;47(2):192–195. doi: 10.1104/pp.47.2.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ryser H. J. A membrane effect of basic polymers dependent on molecular size. Nature. 1967 Aug 26;215(5104):934–936. doi: 10.1038/215934a0. [DOI] [PubMed] [Google Scholar]
  9. Siegel S. M., Daly O. Regulation of betacyanin efflux from beet root by poly-L-lysine, ca-ion and other substances. Plant Physiol. 1966 Nov;41(9):1429–1434. doi: 10.1104/pp.41.9.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Walker-Simmons M., Hadwiger L., Ryan C. A. Chitosans and pectic polysaccharides both induce the accumulation of the antifungal phytoalexin pisatin in pea pods and antinutrient proteinase inhibitors in tomato leaves. Biochem Biophys Res Commun. 1983 Jan 14;110(1):194–199. doi: 10.1016/0006-291x(83)91279-2. [DOI] [PubMed] [Google Scholar]
  11. Young D. H., Köhle H., Kauss H. Effect of Chitosan on Membrane Permeability of Suspension-Cultured Glycine max and Phaseolus vulgaris Cells. Plant Physiol. 1982 Nov;70(5):1449–1454. doi: 10.1104/pp.70.5.1449. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES