Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1983 Dec;73(4):902–905. doi: 10.1104/pp.73.4.902

ATP-Dependent Proteolytic Activity from Spinach Leaves 1

John B W Hammond 1,2, Jack Preiss 1
PMCID: PMC1066577  PMID: 16663340

Abstract

Spinach (Spinacia oleracea CV Bloomsdale Long Standing) leaf cytoplasmic starch phosphorylase and rabbit muscle phosphorylase a were inactivated by incubation with partially purified leaf extract in the presence of ATP and Mg2+. The inactivating factor(s) were heat stable and susceptible to protease attack. Phosphorylase inactivation was prevented by incubation in the presence of p-aminobenzamidine and phenylboronic acid, or prolonged treatment with phenylmethylsulfonyl fluoride or leupeptin for the ATP-stimulated inhibitory activity. Mg2+ -dependent inactivation was prevented by incubation with leupeptin, phenylmethylsulfonyl fluoride, p-aminobenzamidine, or 5′-adenylate. ATP-mediated inactivation of phosphorylase was stimulated by Mg2+ with a reduction in the apparent Km for ATP. Casein-degrading activities with the same properties of ATP and/or Mg2+ stimulation, heat stability, and susceptibility to proteinase inhibitors were detected suggesting that phorphorylase inactivation was due to proteolysis. The activity was greatest at about the time of flowering and also appeared to depend on the light regime.

Full text

PDF
902

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Charette M. F., Henderson G. W., Markovitz A. ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4728–4732. doi: 10.1073/pnas.78.8.4728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Desautels M., Goldberg A. L. Liver mitochondria contain an ATP-dependent, vanadate-sensitive pathway for the degradation of proteins. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1869–1873. doi: 10.1073/pnas.79.6.1869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hammond J. B., Preiss J. Spinach Leaf Intra and Extra Chloroplast Phosphorylase Activities during Growth. Plant Physiol. 1983 Nov;73(3):709–712. doi: 10.1104/pp.73.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hershko A., Ciechanover A., Heller H., Haas A. L., Rose I. A. Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1783–1786. doi: 10.1073/pnas.77.4.1783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hershko A., Ciechanover A. Mechanisms of intracellular protein breakdown. Annu Rev Biochem. 1982;51:335–364. doi: 10.1146/annurev.bi.51.070182.002003. [DOI] [PubMed] [Google Scholar]
  6. Jentoft N., Dearborn D. G. Labeling of proteins by reductive methylation using sodium cyanoborohydride. J Biol Chem. 1979 Jun 10;254(11):4359–4365. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Larimore F. S., Waxman L., Goldberg A. L. Studies of the ATP-dependent proteolytic enzyme, protease La, from Escherichia coli. J Biol Chem. 1982 Apr 25;257(8):4187–4195. [PubMed] [Google Scholar]
  9. Miller B. L., Huffaker R. C. Hydrolysis of Ribulose-1,5-bisphosphate Carboxylase by Endoproteinases from Senescing Barley Leaves. Plant Physiol. 1982 Jan;69(1):58–62. doi: 10.1104/pp.69.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Preiss J., Okita T. W., Greenberg E. Characterization of the spinach leaf phosphorylases. Plant Physiol. 1980 Nov;66(5):864–869. doi: 10.1104/pp.66.5.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ragster L. V., Chrispeels M. J. Azocoll-digesting Proteinases in Soybean Leaves: Characteristics and Changes during Leaf Maturation and Senescence. Plant Physiol. 1979 Nov;64(5):857–862. doi: 10.1104/pp.64.5.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wittenbach V. A. Ribulose Bisphosphate Carboxylase and Proteolytic Activity in Wheat Leaves from Anthesis through Senescence. Plant Physiol. 1979 Nov;64(5):884–887. doi: 10.1104/pp.64.5.884. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES