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. 1984 Jan;74(1):32–38. doi: 10.1104/pp.74.1.32

Ethylene-Independent and Ethylene-Dependent Biochemical Changes in Ripening Tomatoes

David Jeffery 1,2,3,1, Christopher Smith 1,2,3,1, Peter Goodenough 1,2,3, Ian Prosser 1,2,3, Donald Grierson 1,2,3
PMCID: PMC1066619  PMID: 16663381

Abstract

Fruits of Lycopersicon esculentum Mill cv Sonatine stored in 6% CO2, 6% O2, and 88% N2 for 14 weeks at 12°C, exhibited a temporal separation of certain biochemical events associated with ripening.

The specific activity of two citric acid cycle enzymes, citrate synthase and malate dehydrogenase, fell substantially during the first 2 weeks of storage when changes in organic acid concentration also occurred. During this period, lycopene, polygalacturonase, and ethylene were undetectable.

When fruit were removed from store, ethylene was evolved and polygalacturonase and invertase activity were rapidly initiated as was synthesis of lycopene.

To determine whether the changes in organic acid metabolism were affected by ethylene, fruit was kept at 22°C in either a normal atmosphere or a normal atmosphere supplemented with 27 microliters per liter of ethylene, and it was shown that in both atmospheres similar quantitative changes to those described above occurred in the citric acid cycle enzymes specific activities before any detectable increase in the specific activities of invertase and polygalacturonase. These latter changes, together with pigment changes, occurred between 2 and 3 days earlier in fruit exposed to ethylene, compared with those kept in a normal atmosphere.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Chalmers D. J., Rowan K. S. The climacteric in ripening tomato fruit. Plant Physiol. 1971 Sep;48(3):235–240. doi: 10.1104/pp.48.3.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dostal H. C., Leopold A. C. Gibberellin delays ripening of tomatoes. Science. 1967 Dec 22;158(3808):1579–1580. doi: 10.1126/science.158.3808.1579. [DOI] [PubMed] [Google Scholar]
  4. Doyle W. P., Huff R., Wang C. H. Role of Glyoxylate in Biosynthesis of Acids in Tomato Fruit. Plant Physiol. 1960 Sep;35(5):745–750. doi: 10.1104/pp.35.5.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hobson G. E. Polygalacturonase in normal and abnormal tomato fruit. Biochem J. 1964 Aug;92(2):324–332. doi: 10.1042/bj0920324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Themmen A. P., Tucker G. A., Grierson D. Degradation of isolated tomato cell walls by purified polygalacturonase in vitro. Plant Physiol. 1982 Jan;69(1):122–124. doi: 10.1104/pp.69.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tucker G. A., Robertson N. G., Grierson D. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit. Eur J Biochem. 1980 Nov;112(1):119–124. doi: 10.1111/j.1432-1033.1980.tb04993.x. [DOI] [PubMed] [Google Scholar]
  9. Tucker G. A., Robertson N. G., Grierson D. The conversion of tomato-fruit polygalacturonase isoenzyme 2 into isoenzyme 1 in vitro. Eur J Biochem. 1981 Mar 16;115(1):87–90. doi: 10.1111/j.1432-1033.1981.tb06201.x. [DOI] [PubMed] [Google Scholar]

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