Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Jan;74(1):189–191. doi: 10.1104/pp.74.1.189

Pyruvate Orthophosphate Dikinase

Intracellular Site of Synthesis in Maize Leaf Cells 1

Sherry L Gee 1,2, Steven Ruzin 1,2, James A Bassham 1,2
PMCID: PMC1066649  PMID: 16663378

Abstract

Pyruvate orthophosphate dikinase is synthesized in non-green leaf cells of the maize mutant iojap. Since iojap plastids lack ribosomes, it is concluded that the site of synthesis of pyruvate orthophosphate dikinase in maize leaf cells is on ribosomes in the cytoplasm.

Full text

PDF
189

Images in this article

190Fig. 1
on p.190
190Fig. 2
on p.190

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barraclough R., Ellis R. J. The biosynthesis of ribulose bisphosphate carboxylase. Uncoupling of the synthesis of the large and small subunits in isolated soybean leaf cells. Eur J Biochem. 1979 Feb 15;94(1):165–177. doi: 10.1111/j.1432-1033.1979.tb12883.x. [DOI] [PubMed] [Google Scholar]
  2. Blair G. E., Ellis R. J. Protein synthesis in chloroplasts. I. Light-driven synthesis of the large subunit of fraction I protein by isolated pea chloroplasts. Biochim Biophys Acta. 1973 Aug 24;319(2):223–234. doi: 10.1016/0005-2787(73)90013-0. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  5. Hague D. R., Uhler M., Collins P. D. Cloning of cDNA for pyruvate, Pi dikinase from maize leaves. Nucleic Acids Res. 1983 Jul 25;11(14):4853–4865. doi: 10.1093/nar/11.14.4853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hatch M. D., Slack C. R. A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis. Biochem J. 1968 Jan;106(1):141–146. doi: 10.1042/bj1060141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huber S. C., Hall T. C., Edwards G. E. Differential Localization of Fraction I Protein between Chloroplast Types. Plant Physiol. 1976 May;57(5):730–733. doi: 10.1104/pp.57.5.730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  9. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  10. Schmidt S., Reich R., Witt H. T. Electrochromism of chlorophylls and carotenoids in multilayers and in chloroplasts. Naturwissenschaften. 1971 Aug;58(8):414–414. doi: 10.1007/BF00591523. [DOI] [PubMed] [Google Scholar]
  11. Sugiyama T. Occurrence of Pyruvate Orthophosphate Dikinase in the Succulent Plant, Kalanchoë daigremontiana Hamet. et. Perr. Plant Physiol. 1975 Nov;56(5):605–607. doi: 10.1104/pp.56.5.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sugiyama T. Purification, molecular, and catalytic properties of pyruvate phosphate dikinase from the maize leaf. Biochemistry. 1973 Jul 17;12(15):2862–2868. doi: 10.1021/bi00739a014. [DOI] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walbot V., Coe E. H. Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2760–2764. doi: 10.1073/pnas.76.6.2760. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES