Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Feb;74(2):204–207. doi: 10.1104/pp.74.2.204

Occurrence of Isóenzymes of Glutamine Synthetase in the Alga Chlorella kessleri1

Nazira Sumar 1, Peter J Casselton 1, Sheila F McNally 1,2, George R Stewart 1
PMCID: PMC1066655  PMID: 16663398

Abstract

Two forms (GS1 and GS2) of glutamine synthetase have been isolated, separated by ion exchange chromatography, and partly characterized from cells of the green alga Chlorella kessleri. Both forms are present in cells grown autotrophically or heterotrophically on various nitrogen sources, but under all nutritional conditions GS1 was found to be the major isoenzyme present (60-80%). The activity of both isoenzymes was greatest in cells grown under nitrogen-limiting conditions. Both isoenzymes have molecular weights in the range 340 to 350,000 daltons. GS1 was found to have a greater thermostability than GS2: GS1 was stable at 30°C while GS2 lost 95% of its activity in 30 minutes. GS1 was much less sensitive to thiol reactive reagents than GS2.

Full text

PDF
204

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Darrow R. A., Knotts R. R. Two forms of glutamine synthetase in free-living root-nodule bacteria. Biochem Biophys Res Commun. 1977 Sep 23;78(2):554–559. doi: 10.1016/0006-291x(77)90214-5. [DOI] [PubMed] [Google Scholar]
  3. Hirel B., Gadal P. Glutamine Synthetase in Rice: A COMPARATIVE STUDY OF THE ENZYMES FROM ROOTS AND LEAVES. Plant Physiol. 1980 Oct;66(4):619–623. doi: 10.1104/pp.66.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jacobson L. MAINTENANCE OF IRON SUPPLY IN NUTRIENT SOLUTIONS BY A SINGLE ADDITION OF FERRIC POTASSIUM ETHYLENEDIAMINE TETRA-ACETATE. Plant Physiol. 1951 Apr;26(2):411–413. doi: 10.1104/pp.26.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mann A. F., Fentem P. A., Stewart G. R. Identification of two forms of glutamine synthetase in barley (Hordeum vulgare). Biochem Biophys Res Commun. 1979 May 28;88(2):515–521. doi: 10.1016/0006-291x(79)92078-3. [DOI] [PubMed] [Google Scholar]
  6. Mann A. F., Fentem P. A., Stewart G. R. Tissue localization of barley (Hordeum vulgare) glutamine synthetase isoenzymes. FEBS Lett. 1980 Feb 11;110(2):265–267. doi: 10.1016/0014-5793(80)80088-3. [DOI] [PubMed] [Google Scholar]
  7. McNally S. F., Hirel B., Gadal P., Mann A. F., Stewart G. R. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf. Plant Physiol. 1983 May;72(1):22–25. doi: 10.1104/pp.72.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stasiewicz S., Dunham V. L. Isolation and characterization of two forms of glutamine synthetsae from soybean hypocotyl. Biochem Biophys Res Commun. 1979 Mar 30;87(2):627–634. doi: 10.1016/0006-291x(79)91840-0. [DOI] [PubMed] [Google Scholar]
  9. Tischner R. Regulation of Glutamine Synthetase by Light and during Nitrogen Deficiency in Synchronous Chlorella sorokiniana. Plant Physiol. 1980 Nov;66(5):805–808. doi: 10.1104/pp.66.5.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wedler F. C., Kenney R. M., Ashour A. E., Carfi J. Two regulatory isozymes of glutamine synthetase from Bacillus caldolyticus, an extreme thermophile. Biochem Biophys Res Commun. 1978 Mar 15;81(1):122–126. doi: 10.1016/0006-291x(78)91638-8. [DOI] [PubMed] [Google Scholar]
  11. Winter H. C., Powell G. K., Dekker E. E. Glutamine Synthetase of Germinating Peanuts : PROPERTIES OF TWO CHROMATOGRAPHICALLY DISTINCT FORMS AND THEIR ACTIVITY TOWARD 4-METHYLENEGLUTAMIC ACID. Plant Physiol. 1982 Jan;69(1):41–47. doi: 10.1104/pp.69.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES