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. 1984 Feb;74(2):268–271. doi: 10.1104/pp.74.2.268

Calcium Inhibits Phase Shifting of the Circadian Conidiation Rhythm of Neurospora crassa by the Calcium Ionophore A23187 1

Hideaki Nakashima 1
PMCID: PMC1066667  PMID: 16663409

Abstract

Effects of the calcium ionophore, A23187, and antimycin A on the circadian conidiation rhythm of Neurospora crassa were examined. A23187 at a concentration of 1 μm in medium not containing divalent cations delayed the phase by 10 hours at CT 10 and advanced it by 5 hours at CT 14 (CT 12 corresponds to the time that discs are transferred from light to dark). This phase shifting was completely inhibited by addition of 0.1 millimolar CaCl2 but not by MgCl2 at any concentrations examined.

Antimycin A inhibited respiration by 90% at a concentration of 0.2 micrograms per milliliter and lowered the ATP content by 85%. Antimycin A alone caused small phase advances but in combination with A23187 resulted in a large phase delay at CT 10. This phase shifting was not reversed by addition of CaCl2 lower than 10 millimolar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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