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. 1984 Feb;74(2):448–450. doi: 10.1104/pp.74.2.448

Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L

Bertrand Hirel 1,2, Claire Weatherley 1,2, Claude Cretin 1,2, Catherine Bergounioux 1,2, Pierre Gadal 1,2
PMCID: PMC1066701  PMID: 16663441

Abstract

Chloroplastic glutamine synthetase from tobacco leaves (Nicotiana tabacum L. var Xanthi) was purified to homogeneity. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography, a single subunit was identified with a molecular weight of 45,000 daltons. However the native protein seems to be composed of four different subunits which can be separated by isoelectrofocusing. It is suggested that different genes with eventual posttranslational and/or posttranscriptional modifications may control the synthesis of the chloroplastic glutamine synthetase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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