Abstract
The analysis of Sha'afi et al. (Sha'afi, Rich, Mickulecky, Solomon 1970 J Gen Physiol 55: 427-450) for determining solute permeability in red blood cells has been modified and applied to turgid plant cells. Following the addition of permeating solute to the external medium, a biphasic response of cell turgor can be measured with the pressure probe in isolated internodes of Chara corallina. After an initial decrease in turgor due to water flow (water phase), turgor increases due to the uptake of the solute (solute phase) until the original turgor is reattained. From the pressure/time course in the neighborhood of the minimum turgor, the permeability of the osmotic solute can be determined. The data obtained by the minimum method for rapidly permeating (ethanol, methanol) and slowly permeating (formamide, dimethylformamide) solutes are similar to those calculated from the half-time of pressure changes during the solute phase and to data obtained by other workers using radioactive tracers. The methods employing the pressure probe were applied to examine the effect of high pH (up to pH 11) on the membrane permeability. There appeared to be no effect of high pH on the permeability coefficients, reflection coefficients, and hydraulic conductivity.
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Selected References
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- DAINTY J., GINZBURG B. Z. THE PERMEABILITY OF THE PROTOPLASTS OF CHARA AUSTRALIS AND NITELLA TRANSLUCENS TO METHANOL, ETHANOL AND ISOPROPANOL. Biochim Biophys Acta. 1964 Jan 27;79:122–128. doi: 10.1016/0926-6577(64)90045-2. [DOI] [PubMed] [Google Scholar]
- DAINTY J., GINZBURG B. Z. THE REFLECTION COEFFICIENT OF PLANT CELL MEMBRANES FOR CERTAIN SOLUTES. Biochim Biophys Acta. 1964 Jan 27;79:129–137. doi: 10.1016/0926-6577(64)90046-4. [DOI] [PubMed] [Google Scholar]
- Hüsken D., Steudle E., Zimmermann U. Pressure probe technique for measuring water relations of cells in higher plants. Plant Physiol. 1978 Feb;61(2):158–163. doi: 10.1104/pp.61.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mlekoday H. J., Moore R., Levitt D. G. Osmotic water permeability of the human red cell. Dependence on direction of water flow and cell volume. J Gen Physiol. 1983 Feb;81(2):213–220. doi: 10.1085/jgp.81.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sha'afi R. I., Rich G. T., Mikulecky D. C., Solomon A. K. Determination of urea permeability in red cells by minimum method. A test of the phenomenological equations. J Gen Physiol. 1970 Apr;55(4):427–450. doi: 10.1085/jgp.55.4.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wendler S., Zimmermann U. A new method for the determination of hydraulic conductivity and cell volume of plant cells by pressure clamp. Plant Physiol. 1982 May;69(5):998–1003. doi: 10.1104/pp.69.5.998. [DOI] [PMC free article] [PubMed] [Google Scholar]