Fig. 5.
Effect of chemotherapy on PCTS: changes in tumour cell viability and proliferation are detected in tumour slices in response to doxorubicin treatment. Untreated tumour slices (black) and PCTS exposed to 2 μM doxorubicin (red, Doxo) were collected at the indicated timepoints and the following parameters analysed for npt = 2. a | LDH release in the supernatants, shown as OD normalised per slice weight, ratio untreated vs doxo; mean ± SD, nPCTS = 6. b | Adenylate energy charge (AEC) and ATP/mg tissue in a representative slice per timepoint from pt 168. c | Cell proliferation by evaluation of Ki67 staining; mean ± SD, nPCTS = 6. d | Apoptosis by quantification of TUNEL positive nuclei; mean ± SD, nPCTS >6. e | Histological evaluation of H&E staining; nPCTS = 6. f | Stroma analysis of haematoxylin and Sirius red staining; nPCTS = 6. c–f | Representative images are from pt 168, please note some images have been seen in the summary Fig. 3. Scale bars: 100 μm.