Abstract
Hydrogen and carbon isotope ratios of cellulose nitrate and oxygen isotope ratios of cellulose from C3, C4, and Crassulacean acid metabolism (CAM) plants were determined for plants growing within a small area in Val Verde County, Texas. Plants having CAM had distinctly higher deuterium/hydrogen (D/H) ratios than plants having C3 and C4 metabolism. When hydrogen isotope ratios are plotted against carbon isotope ratios, each photosynthetic mode separates into a distinct cluster of points. C4 plants had many D/H ratios similar to those of C3 plants, so that hydrogen isotope ratios cannot be used to distinguish between these two photosynthetic modes. Portulaca mundula, which may have a modified photosynthetic mode between C4 and CAM, had a hydrogen isotope ratio between those of the C4 and CAM plants. When oxygen isotope ratios are plotted against carbon isotope ratios, no distinct clustering of the C4 and CAM plants occurs. Thus, oxygen isotope ratios are not useful in distinguishing between these metabolic modes. A plot of hydrogen isotope ratios versus oxygen isotope ratios for this sample set shows considerable overlap between oxygen isotope ratios of the different photosynthetic modes without a concomitant overlap in the hydrogen isotope ratios of CAM and the other two photosynthetic modes. This observation is consistent with the hypothesis that higher D/H ratios in CAM plants relative to C3 and C4 plants are due to isotopic fractionations occurring during biochemical reactions.
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Selected References
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- Bender M. M. C/C ratio changes in crassulacean Acid metabolism plants. Plant Physiol. 1973 Nov;52(5):427–430. doi: 10.1104/pp.52.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig H. Isotopic Variations in Meteoric Waters. Science. 1961 May 26;133(3465):1702–1703. doi: 10.1126/science.133.3465.1702. [DOI] [PubMed] [Google Scholar]
- Deniro M. J., Epstein S. Relationship between the oxygen isotope ratios of terrestrial plant cellulose, carbon dioxide, and water. Science. 1979 Apr 6;204(4388):51–53. doi: 10.1126/science.204.4388.51. [DOI] [PubMed] [Google Scholar]
- Koch K., Kennedy R. A. Characteristics of Crassulacean Acid Metabolism in the Succulent C(4) Dicot, Portulaca oleracea L. Plant Physiol. 1980 Feb;65(2):193–197. doi: 10.1104/pp.65.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ku S. B., Shieh Y. J., Reger B. J., Black C. C. Photosynthetic Characteristics of Portulaca grandiflora, a Succulent C(4) Dicot : CELLULAR COMPARTMENTATION OF ENZYMES AND ACID METABOLISM. Plant Physiol. 1981 Nov;68(5):1073–1080. doi: 10.1104/pp.68.5.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PONTICORVO L., RITTENBERG D. A method for the determination of the O18 concentration of the oxygen of organic compounds. Int J Appl Radiat Isot. 1956 Nov;1(3):208–214. doi: 10.1016/0020-708x(56)90008-4. [DOI] [PubMed] [Google Scholar]
- Smith B. N., Epstein S. Biogeochemistry of the stable isotopes of hydrogen and carbon in salt marsh biota. Plant Physiol. 1970 Nov;46(5):738–742. doi: 10.1104/pp.46.5.738. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sternberg L. O., Deniro M. J., Ting I. P. Carbon, hydrogen, and oxygen isotope ratios of cellulose from plants having intermediary photosynthetic modes. Plant Physiol. 1984 Jan;74(1):104–107. doi: 10.1104/pp.74.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sternberg L., Deniro M. J. Isotopic Composition of Cellulose from C3, C4, and CAM Plants Growing Near One Another. Science. 1983 May 27;220(4600):947–949. doi: 10.1126/science.220.4600.947. [DOI] [PubMed] [Google Scholar]