Figure 3.

Examination of sample coverage and overlap within and across sample types. A. Principal component analysis (PCA) of all biological replicates for each of the three sample types. All biological replicates within a treatment group were found to cluster together, indicating reproducibility of our method. B. Box plots showing overall proteome coverage in terms of normalized protein expression levels. Looking across all three treatment groups, the majority of expression levels remain relatively constant against one another, demonstrating that serum replacement and analysis of secreted proteins does not substantially alter protein intensities.