Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Apr;74(4):759–765. doi: 10.1104/pp.74.4.759

Differences between Wheat Genotypes in Specific Activity of Ribulose-1,5-bisphosphate Carboxylase and the Relationship to Photosynthesis

John R Evans 1,1, Jeffrey R Seemann 1,2
PMCID: PMC1066764  PMID: 16663506

Abstract

The in vitro ribulose-1,5-bisphosphate (RuBP) carboxylase activity per unit of leaf nitrogen was found to be 30% greater in Triticum aestivum than in T. monococcum. This was due to a higher specific activity of the enzyme from T. aestivum, as the amount of RuBP carboxylase protein per unit of total leaf nitrogen did not differ between the genotypes. The occurrence of higher specific activity of RuBP carboxylase is shown to correlate with possession of the large subunit derived from the B genome of wheat.

Despite the greater RuBP carboxylase activity per unit of leaf nitrogen in T. aestivum, the initial slopes of curves relating rate of CO2 assimilation to intercellular p(CO2) are similar in T. aestivum and T. monococcum for the same nitrogen content per unit leaf area. The similarity of the initial slopes is the result of a greater resistance to CO2 transfer between the intercellular spaces and the site of carboxylation in T. aestivum than in T. monococcum.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Byrne M. C., Nelson C. J., Randall D. D. Ploidy effects on anatomy and gas exchange of tall fescue leaves. Plant Physiol. 1981 Oct;68(4):891–893. doi: 10.1104/pp.68.4.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean C., Leech R. M. Genome Expression during Normal Leaf Development : 2. Direct Correlation between Ribulose Bisphosphate Carboxylase Content and Nuclear Ploidy in a Polyploid Series of Wheat. Plant Physiol. 1982 Dec;70(6):1605–1608. doi: 10.1104/pp.70.6.1605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evans J. R. Nitrogen and Photosynthesis in the Flag Leaf of Wheat (Triticum aestivum L.). Plant Physiol. 1983 Jun;72(2):297–302. doi: 10.1104/pp.72.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hall N. P., Pierce J., Tolbert N. E. Formation of a carboxyarabinitol bisphosphate complex with ribulose bisphosphate carboxylase/oxygenase and theoretical specific activity of the enzyme. Arch Biochem Biophys. 1981 Nov;212(1):115–119. doi: 10.1016/0003-9861(81)90349-0. [DOI] [PubMed] [Google Scholar]
  5. Joseph M. C., Randall D. D. Photosynthesis in Polyploid Tall Fescue : II. PHOTOSYNTHESIS AND RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE OF POLYPLOID TALL FESCUE. Plant Physiol. 1981 Oct;68(4):894–898. doi: 10.1104/pp.68.4.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McNeil P. H., Foyer C. H., Walker D. A. Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars. Plant Physiol. 1981 Mar;67(3):530–534. doi: 10.1104/pp.67.3.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meyers S. P., Brinegar A. C., Schrader L. E., Jordan D. B., Ogren W. L. Ploidy Effects in Isogenic Populations of Alfalfa (Medicago sativa L.) : IV. Similarity in Physical and Kinetic Properties of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase. Plant Physiol. 1983 Apr;71(4):966–968. doi: 10.1104/pp.71.4.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meyers S. P., Nichols S. L., Baer G. R., Molin W. T., Schrader L. E. Ploidy effects in isogenic populations of alfalfa : I. Ribulose-1,5-bisphosphate carboxylase, soluble protein, chlorophyll, and DNA in leaves. Plant Physiol. 1982 Dec;70(6):1704–1709. doi: 10.1104/pp.70.6.1704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Paech C., Pierce J., McCurry S. D., Tolbert N. E. Inhibition of ribulose-1,5-biphosphate carboxylase/oxygenase by ribulose-1,5-bisphosphate epimerization and degradation products. Biochem Biophys Res Commun. 1978 Aug 14;83(3):1084–1092. doi: 10.1016/0006-291x(78)91506-1. [DOI] [PubMed] [Google Scholar]
  10. Rejda J. M., Johal S., Chollet R. Enzymic and physicochemical characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from diploid and tetraploid cultivars of perennial ryegrass. Arch Biochem Biophys. 1981 Sep;210(2):617–624. doi: 10.1016/0003-9861(81)90228-9. [DOI] [PubMed] [Google Scholar]
  11. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yeoh H. H., Badger M. R., Watson L. Variations in K(m)(CO(2)) of Ribulose-1,5-bisphosphate Carboxylase among Grasses. Plant Physiol. 1980 Dec;66(6):1110–1112. doi: 10.1104/pp.66.6.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES