Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1984 May;75(1):222–224. doi: 10.1104/pp.75.1.222

Evidence for the Occurrence of, and Possible Physiological Role for, Cyanobacterial Calmodulin 1

Geoffrey W Kerson 1, Jan A Miernyk 1, Kenneth Budd 1
PMCID: PMC1066866  PMID: 16663576

Abstract

Phosphate uptake by the blue-green alga Oscillatoria limnetica Lemmerman is stimulated by micromolar concentrations of Ca2+. The calmodulin antagonists 4-(3-[2(trifluoromethyl)phenylthiazin-10-yl]propyl)-1-piperazine ethanol-HCl and its monofluoro-analog inhibit orthophosphate uptake of Oscillatoria limnetica by over 97% implying involvement of calmodulin in this process. A calmodulin-like protein was quantitated in cell-free extracts from O. limnetica by radioimmunoassay.

Full text

PDF
222

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr R., Troxel K. S., Crane F. L. Calmodulin antagonists inhibit electron transport in photosystem II of spinach chloroplasts. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1182–1188. doi: 10.1016/0006-291x(82)91375-4. [DOI] [PubMed] [Google Scholar]
  2. Cheung W. Y. Calmodulin plays a pivotal role in cellular regulation. Science. 1980 Jan 4;207(4426):19–27. doi: 10.1126/science.6243188. [DOI] [PubMed] [Google Scholar]
  3. HUGHES E. O., GORHAM P. R., ZEHNDER A. Toxicity of a unialgal culture of Microcystis aeruginosa. Can J Microbiol. 1958 Jun;4(3):225–236. doi: 10.1139/m58-024. [DOI] [PubMed] [Google Scholar]
  4. Iwasa Y., Yonemitsu K., Matsui K., Fukunaga K., Miyamoto E. Calmodulin-like activity in the soluble fraction of Escherichia coli. Biochem Biophys Res Commun. 1981 Feb 12;98(3):656–660. doi: 10.1016/0006-291x(81)91164-5. [DOI] [PubMed] [Google Scholar]
  5. Jamieson G. A., Jr, Bronson D. D., Schachat F. H., Vanaman T. C. Structure and function relationships among calmodulins and troponin C-like proteins from divergent eukaryotic organisms. Ann N Y Acad Sci. 1980;356:1–13. doi: 10.1111/j.1749-6632.1980.tb29593.x. [DOI] [PubMed] [Google Scholar]
  6. Jarrett H. W., Brown C. J., Black C. C., Cormier M. J. Evidence that calmodulin is in the chloroplast of peas and serves a regulatory role in photosynthesis. J Biol Chem. 1982 Nov 25;257(22):13795–13804. [PubMed] [Google Scholar]
  7. Kurn N. Inhibition of phosphate uptake by fluphenazine, a calmodulin inhibitor. Analysis of Volvox wild-type and fluphenazine-resistant mutant strains. FEBS Lett. 1982 Jul 19;144(1):68–72. doi: 10.1016/0014-5793(82)80570-x. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Levin R. M., Weiss B. Specificity of the binding of trifluoperazine to the calcium-dependent activator of phosphodiesterase and to a series of other calcium-binding proteins. Biochim Biophys Acta. 1978 May 3;540(2):197–204. doi: 10.1016/0304-4165(78)90132-0. [DOI] [PubMed] [Google Scholar]
  10. Lin W. Potassium and Phosphate Uptake in Corn Roots: Further Evidence for an Electrogenic H/K Exchanger and an OH/Pi Antiporter. Plant Physiol. 1979 May;63(5):952–955. doi: 10.1104/pp.63.5.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roomans G. M., Borst-Pauwels G. W. Interaction of cations with phosphate uptake by Saccharomyces cerevisiae. Effects of surface potential. Biochem J. 1979 Mar 15;178(3):521–527. doi: 10.1042/bj1780521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg H., Gerdes R. G., Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977 Aug;131(2):505–511. doi: 10.1128/jb.131.2.505-511.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van Eldik L. J., Piperno G., Watterson D. M. Comparative biochemistry of calmodulins and calmodulin-like proteins. Ann N Y Acad Sci. 1980;356:36–42. doi: 10.1111/j.1749-6632.1980.tb29597.x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES