Abstract
Though Helminthosporium maydis race T (HmT) toxin decreased active Ca2+ uptake into mitochondria isolated from susceptible (T) but not resistant (N) corn (Kimber, Sze, 1984 Plant Physiol 74: 804-809 the mode of toxin action is not understood. This study shows that HmT toxin or A23187 (a Ca2+ ionophore) dissipated a Ca2+ gradient in T mitochondria. However, HmT toxin had no effect on Ca2+ gradients in N mitochondria or microsomal vesicles from T or N corn. The results suggest that HmT toxin increased membrane permeability to Ca2+ in mitochondria of T corn specifically.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Akerman K. E., Moore A. L. Phosphate dependent, ruthenium red insensitive CA2+ uptake in mung bean mitochondria. Biochem Biophys Res Commun. 1983 Aug 12;114(3):1176–1181. doi: 10.1016/0006-291x(83)90686-1. [DOI] [PubMed] [Google Scholar]
- Bednarski M. A., Izawa S., Scheffer R. P. Reversible Effects of Toxin from Helminthosporium maydis Race T on Oxidative Phosphorylation by Mitochondria from Maize. Plant Physiol. 1977 Apr;59(4):540–545. doi: 10.1104/pp.59.4.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Kimber A., Sze H. Helminthosporium maydis T Toxin Decreased Calcium Transport into Mitochondria of Susceptible Corn. Plant Physiol. 1984 Apr;74(4):804–809. doi: 10.1104/pp.74.4.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. J., Koeppe D. E. Southern corn leaf blight: susceptible and resistant mitochondria. Science. 1971 Jul 2;173(3991):67–69. doi: 10.1126/science.173.3991.67. [DOI] [PubMed] [Google Scholar]
- Mitchell P. The Ninth Sir Hans Krebs Lecture. Compartmentation and communication in living systems. Ligand conduction: a general catalytic principle in chemical, osmotic and chemiosmotic reaction systems. Eur J Biochem. 1979 Mar 15;95(1):1–20. doi: 10.1111/j.1432-1033.1979.tb12934.x. [DOI] [PubMed] [Google Scholar]
- Nicholls D., Akerman K. Mitochondrial calcium transport. Biochim Biophys Acta. 1982 Sep 1;683(1):57–88. doi: 10.1016/0304-4173(82)90013-1. [DOI] [PubMed] [Google Scholar]
- Payne G., Kono Y., Daly J. M. A Comparison of Purified Host Specific Toxin from Helminthosporium maydis, Race T, and Its Acetate Derivative on Oxidation by Mitochondria from Susceptible and Resistant Plants. Plant Physiol. 1980 May;65(5):785–791. doi: 10.1104/pp.65.5.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sze H. Nigericin-stimulated ATPase activity in microsomal vesicles of tobacco callus. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5904–5908. doi: 10.1073/pnas.77.10.5904. [DOI] [PMC free article] [PubMed] [Google Scholar]
