Abstract
Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-β-1,4-glucanase purified from the culture fluid of Trichoderma viride. A nonasaccharide-rich Bio-Gel P-2 fraction of this digest inhibited 2,4-dichlorophenoxyacetic-acid-stimulated elongation of etiolated pea stem segments. The inhibitory activity of this oligosaccharide fraction exhibited a well-defined concentration optimum between 10−2 and 10−1 micrograms per milliliter. Another fraction of the same xyloglucan digest, rich in a structurally related heptasaccharide, did not, at similar concentrations, significantly inhibit the elongation.
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Selected References
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- Bauer W. D., Talmadge K. W., Keegstra K., Albersheim P. The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells. Plant Physiol. 1973 Jan;51(1):174–187. doi: 10.1104/pp.51.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labavitch J. M., Ray P. M. Turnover of cell wall polysaccharides in elongating pea stem segments. Plant Physiol. 1974 May;53(5):669–673. doi: 10.1104/pp.53.5.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noodén L. D., Thimann K. V. EVIDENCE FOR A REQUIREMENT FOR PROTEIN SYNTHESIS FOR AUXIN-INDUCED CELL ENLARGEMENT. Proc Natl Acad Sci U S A. 1963 Aug;50(2):194–200. doi: 10.1073/pnas.50.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terry M. E., Jones R. L. Soluble Cell Wall Polysaccharides Released from Pea Stems by Centrifugation : I. EFFECT OF AUXIN. Plant Physiol. 1981 Sep;68(3):531–537. doi: 10.1104/pp.68.3.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valent B. S., Darvill A. G., McNeil M., Robertsen B. K., Albersheim P. A general and sensitive chemical method for sequencing the glycosyl residues of complex carbohydrates. Carbohydr Res. 1980 Mar;79(2):165–192. doi: 10.1016/s0008-6215(00)83830-6. [DOI] [PubMed] [Google Scholar]
- Verma D. P., Maclachlan G. A., Byrne H., Ewings D. Regulation and in vitro translation of messenger ribonucleic acid for cellulase from auxin-treated pea epicotyls. J Biol Chem. 1975 Feb 10;250(3):1019–1026. [PubMed] [Google Scholar]