Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1984 Jun;75(2):349–353. doi: 10.1104/pp.75.2.349

Auxin-Regulated Polypeptide Changes at Different Stages of Strawberry Fruit Development 1

K Veluthambi 1, B W Poovaiah 1
PMCID: PMC1066910  PMID: 16663624

Abstract

The pattern of polypeptides at different stages of strawberry (Fragaria ananassa Duch. cv Ozark Beauty) fruit development was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. An 81,000-dalton polypeptide appeared between 5 and 10 days after pollination. Polypeptides with molecular weights of 76,000 and 37,000 daltons were formed after 10 days. The control exerted by auxin in the stage-specific formation of polypeptides was investigated by stopping fruit growth after removing the achenes and reinitiating fruit growth by the application of a synthetic auxin, α-naphthaleneacetic acid (NAA). When the achenes were removed from the 5- and 10-day-old fruits, the fruits failed to grow, the 81,000 dalton polypeptide was not formed between 5 and 10 days, and the 76,000- and 37,000-dalton polypeptides were not formed between 10 and 20 days. Application of NAA to fruits deprived of auxin by removal of achenes resulted in the resumption of growth and also in the appearance of these polypeptides. Removal of achenes of the 5- or 10-day-old fruits and growing them without auxin resulted in the formation of 52,000- and 57,000-dalton polypeptides. These two polypeptides were not formed when NAA was applied to fruits after removal of achenes. Supply of NAA to auxin-deprived fruits 5 days after removal of achenes resulted in resumption of growth and also in the disappearance of these two polypeptides, pointing out their possible relation to the inhibition of fruit growth.

Full text

PDF
349

Images in this article

351Fig. 1
on p.351
352Fig. 2
on p.352
352Fig. 3
on p.352
353Fig. 4
on p.353

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Guilfoyle T. J., Lin C. Y., Chen Y. M., Nagao R. T., Key J. L. Enhancement of soybean RNA polymerase I by auxin. Proc Natl Acad Sci U S A. 1975 Jan;72(1):69–72. doi: 10.1073/pnas.72.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Key J. L., Barnett N. M., Lin C. Y. RNA and protein biosynthesis and the regulation of cell elongation by auxin. Ann N Y Acad Sci. 1967 Aug 9;144(1):49–62. doi: 10.1111/j.1749-6632.1967.tb34000.x. [DOI] [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Leong C. C., Shen T. C. Occurrence of nitrate reductase inhibitor in rice plants. Plant Physiol. 1982 Dec;70(6):1762–1763. doi: 10.1104/pp.70.6.1762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Narayanan K. R., Mudge K. W., Poovaiah B. W. Demonstration of auxin binding to strawberry fruit membranes. Plant Physiol. 1981 Dec;68(6):1289–1293. doi: 10.1104/pp.68.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Noodén L. D., Thimann K. V. EVIDENCE FOR A REQUIREMENT FOR PROTEIN SYNTHESIS FOR AUXIN-INDUCED CELL ENLARGEMENT. Proc Natl Acad Sci U S A. 1963 Aug;50(2):194–200. doi: 10.1073/pnas.50.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ray P. M. Regulation of beta-Glucan Synthetase Activity by Auxin in Pea Stem Tissue: II. Metabolic Requirements. Plant Physiol. 1973 Apr;51(4):609–614. doi: 10.1104/pp.51.4.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Theologis A., Ray P. M. Early auxin-regulated polyadenylylated mRNA sequences in pea stem tissue. Proc Natl Acad Sci U S A. 1982 Jan;79(2):418–421. doi: 10.1073/pnas.79.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vanderhoef L. N., Stahl C. A., Lu T. Y. Two elongation responses to auxin respond differently to protein synthesis inhibition. Plant Physiol. 1976 Sep;58(3):402–404. doi: 10.1104/pp.58.3.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Verma D. P., Maclachlan G. A., Byrne H., Ewings D. Regulation and in vitro translation of messenger ribonucleic acid for cellulase from auxin-treated pea epicotyls. J Biol Chem. 1975 Feb 10;250(3):1019–1026. [PubMed] [Google Scholar]
  11. Walker J. C., Key J. L. Isolation of cloned cDNAs to auxin-responsive poly(A)RNAs of elongating soybean hypocotyl. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7185–7189. doi: 10.1073/pnas.79.23.7185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zurfluh L. L., Guilfoyle T. J. Auxin-induced changes in the patterns of protein synthesis in soybean hypocotyl. Proc Natl Acad Sci U S A. 1980 Jan;77(1):357–361. doi: 10.1073/pnas.77.1.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zurfluh L. L., Guilfoyle T. J. Auxin-induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyl. Plant Physiol. 1982 Feb;69(2):332–337. doi: 10.1104/pp.69.2.332. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES