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. 1984 Jun;75(2):466–473. doi: 10.1104/pp.75.2.466

Sulfate Uptake and Its Regulation in Lemna paucicostata Hegelm. 6746

Anne H Datko 1,1, S Harvey Mudd 1
PMCID: PMC1066931  PMID: 16663645

Abstract

The results of studies of SO42− uptake by Lemna paucicostata are most simply interpreted by the hypothesis that at least two components are involved, one saturating and one linear, `nonsaturating.' The saturating component has a low Km and high specificity for SO42−. Uptake by the nonsaturating component is less affected by pH and temperature than is that of the saturating system. SO42− efflux is not quantitatively important in Lemna under standard conditions (20 micromolar SO42−) (Datko AH, SH Mudd 1980 Plant Physiol 65: 906-912). 55% of newly taken up 35SO42− enters a slowly turning over compartment (vacuole?); 45% remains in a compartment (cytoplasm?) in which it is rapidly metabolized to organic compounds.

Growth in increased concentrations of SO42− or cystine, but not methionine, down-regulates the saturating, but not the nonsaturating, system. Growth in limiting SO42− up-regulates the saturating system. Overall, a 500-fold change was observed. Reciprocal inhibition experiments demonstrated that molybdate and SO42− are taken up by a common mechanism, but growth in molybdate failed to up-regulate SO42− uptake. Regulation by growth in SO42− or cystine did not markedly affect uptake of phosphate or of several organic compounds.

The saturating system contributes 99% of SO42− uptake under standard conditions, providing sufficient SO42− so it is not limiting. In nature the same system likely contributes at least 65 to 70%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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