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. 1984 Jul;75(3):561–565. doi: 10.1104/pp.75.3.561

Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate 1

III. Properties of the Cytosolic Fructose 1,6-Bisphosphatase

Bernd Herzog 1, Mark Stitt 1, Hans W Heldt 1
PMCID: PMC1066955  PMID: 16663666

Abstract

The cytosolic fructose 1,6-bisphosphatase from spinach (Spinacia oleracea U.S. hybrid 424) leaves has been partially purified and its response to fructose 2,6-bisphosphate, AMP, and fructose 1,6-bisphosphate studied, using concentrations present in the cytosol during photosynthesis. In the presence of fructose 2,6-bisphosphate, the substrate saturation kinetics for fructose 1,6-bisphosphate are sigmoidal, with half-maximal activity being attained in 0.1 to 1 millimolar concentration range. The inhibition is enhanced by AMP. Using these results, and information published elsewhere on metabolite concentrations, it is discussed how fructose 1,6-bisphosphatase activity will vary in vivo in response to alterations in the availability of triose phosphate and AMP, and the accumulation of the product, fructose 6-phosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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