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. 1984 Jul;75(3):639–644. doi: 10.1104/pp.75.3.639

Heat Shock Proteins in Tobacco Cell Suspension during Growth Cycle 1

Jan Kanabus 1, Craig S Pikaard 1, Joe H Cherry 1
PMCID: PMC1066968  PMID: 16663679

Abstract

Tobacco (Nicotiana tabacum L. cv Wisconsin 38) cells grown in suspension culture at 26°C produce heat shock proteins (HSPs) when exposed to elevated temperature of 34 to 42°C. At 34 and 38°C, synthesis of normal proteins is maintained while HSPs are expressed within 30 minutes after initiation of the shock. At 42°C, HSPs are still expressed but normal proteins are made at a reduced rate or not at all. Exposure of cells to 38°C allows for a full expression of HSPs without inhibition of the synthesis of normal proteins. Induced synthesis of HSPs at 38°C is maximal 1 to 2 hours after elevation of temperature and diminishes thereafter through at least 6 hours. Cells growing asynchronously in the logarithmic phase of growth produce HSPs at a much higher rate than those in the stationary phase. The ability to synthesize HSPs disappears about one generation time before the cells reach a growth plateau.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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