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. 1984 Jul;75(3):679–687. doi: 10.1104/pp.75.3.679

Physiological Studies on Pea Tendrils 1

XIV. Effects of Mechanical Perturbation, Light, and 2-Deoxy-d-Glucose on Callose Deposition and Tendril Coiling

Terrence E Riehl 1, Mordecai J Jaffe 1
PMCID: PMC1066976  PMID: 16663687

Abstract

When excised tendrils of pea (Pisum sativum L. cv Alaska) are mechanically perturbed there is an immediate and transient increase in callose deposition in the sieve cells. Mechanical perturbation (MP) results in a coiling response in light-grown tendrils and in dark-adapted tendrils, provided, in the latter case, that they receive adequate illumination within a limited period of time after MP. In nonperturbed tendrils the number of callose deposits decreases to some minimum with increasing time in the dark, and their ability to coil in the dark in response to MP diminishes with time in the dark. The transient increase of callose deposition due to MP, however, occurs whether or not tendrils are dark adapted, and whether they receive light or are retained in the dark after MP. This indicates that if callose is directly involved in tendril coiling, then it exerts its effect on the sensory perception of the mechanical stimulus. In the present investigation, there is never tendril coiling without the transient increase in callose, and the time after MP at which the peak of callose deposition occurs precedes the time of the peak amount of coiling.

An inhibitor of callose formation, 2-deoxy-d-glucose (DDG), is equally effective at inhibiting tendril coiling and MP-induced callose deposition, indicating, within the limitations of the specificity of DDG, that callose deposition may be required in order for the coiling response to occur. Alternatively, DDG may prevent the availability of some other factor necessary for tendril coiling.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeles F. B., Bosshart R. P., Forrence L. E., Habig W. H. Preparation and purification of glucanase and chitinase from bean leaves. Plant Physiol. 1971 Jan;47(1):129–134. doi: 10.1104/pp.47.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abeles F. B., Forrence L. E. Temporal and hormonal control of beta-1,3-glucanase in Phaseolus vulgaris L. Plant Physiol. 1970 Apr;45(4):395–400. doi: 10.1104/pp.45.4.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson R. L., Ray P. M. Labeling of the Plasma Membrane of Pea Cells by a Surface-localized Glucan Synthetase. Plant Physiol. 1978 May;61(5):723–730. doi: 10.1104/pp.61.5.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Currier H. B., Webster D. H. Callose Formation and Subsequent Disappearance: Studies in Ultrasound Stimulation. Plant Physiol. 1964 Sep;39(5):843–847. doi: 10.1104/pp.39.5.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jaffe M. J. Experimental separation of sensory and motor functions in pea tendrils. Science. 1977 Jan 14;195(4274):191–192. doi: 10.1126/science.195.4274.191. [DOI] [PubMed] [Google Scholar]
  6. Jaffe M. J., Galston A. W. Physiological Studies on Pea Tendrils. II. The Role of Light and ATP in Contact Coiling. Plant Physiol. 1966 Sep;41(7):1152–1158. doi: 10.1104/pp.41.7.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jaffe M. J., Galston A. W. Physiological studies on pea tendrils. I. Growth and coiling following mechanical stimulation. Plant Physiol. 1966 Jun;41(6):1014–1025. doi: 10.1104/pp.41.6.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jaffe M. J., Galston A. W. Physiological studies on pea tendrils. V. Membrane changes and water movement associated with contact coiling. Plant Physiol. 1968 Apr;43(4):537–542. doi: 10.1104/pp.43.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kivilaan A., Bandurski R. S., Schulze A. A partial characterization of an autolytically solubilized cell wall glucan. Plant Physiol. 1971 Oct;48(4):389–393. doi: 10.1104/pp.48.4.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maresca B., Jaffe B. M., Santoro M. G. Sulfhydryl groups and the differentiation of murine erythroleukemia cells. Biochem Biophys Res Commun. 1979 Dec 14;91(3):1148–1156. doi: 10.1016/0006-291x(79)92000-x. [DOI] [PubMed] [Google Scholar]
  11. McNairn R. B. Phloem Translocation and Heat-induced Callose Formation in Field-grown Gossypium hirsutum L. Plant Physiol. 1972 Sep;50(3):366–370. doi: 10.1104/pp.50.3.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterson C. A. Callose Deposition and Photoassimilate Export in Phaseolus vulgaris Exposed to Excess Cobalt, Nickel, and Zinc. Plant Physiol. 1979 Jun;63(6):1170–1174. doi: 10.1104/pp.63.6.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ulrychová M., Petrů E., Pazourková Z. Permanent staining of callose in plant material by ponceau S. Stain Technol. 1976 Sep;51(5):272–275. doi: 10.3109/10520297609116718. [DOI] [PubMed] [Google Scholar]

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