Volume 64, no. 5, p. 1743–1749, 1998. Page 1745, Table 1: the table should read as shown below.
TABLE 1.
PCR primers and oligonucleotide probes used for IPC construction and detection of Giardia, Cryptosporidium, and IPC
| Oligonucleotide
|
Target | Sequence | PCR product size (bp) | Reference(s) | |
|---|---|---|---|---|---|
| Primer pair or probe | Name | ||||
| Primer pair 1 | GGL | Giardia giardin gene | 5′-AAGTGCGTCAACGAGCAGCT-3′ | 171 | 23, 24 |
| GGR | 5′-TTAGTGCTTTGTGACCATCGA-3′ | ||||
| Primer pair 2 | CHSP1 | C. parvum hsp70 gene | 5′-AGCAATCCTCTGCCGTACAGG-3′ | 590 | 39 |
| CHSP4 | 5′-AAGAGCATCCTTGATCTTCT-3′ | ||||
| Primer pair 3 | IPCF | IPC | 5′-ATGACAGCCACTCCT-3′ | 290 | This study |
| IPCR | 5′-ATGTCAGTTGTGACCACGAA-3′ | ||||
| Primer pair 4 | GHSP1 | Giardia heat shock gene | 5′-AGGGCTCCGGCATAACTTTCC-3′ | 163 | 6 |
| GHSP2 | 5′-GTATCTGTGACCCGTCCGAG-3′ | ||||
| Primer pair 5 | CPHSP2423 | C. parvum hsp70 gene | 5′-AAATGGTGAGCAATCCTCTG-3′ | 361 | 29 |
| CPHSP2764 | 5′-CTTGCTGCTCTTACCAGTAC-3′ | ||||
| Primer pair 6 | T7 promoter-CHSP1 link | IPC | 5′-GGATCCTAATACGACTCACTATAATGACAGCCACTCCT-3′ | 328 | This study |
| Poly(T)15-CHSP4 link | 5′-TTTTTTTTTTTTTTTATGTCAGTTGTGACCACGAA-3′ | ||||
| Primer pair 7 | CPHSP2386 | Cryptosporidium spp. hsp70 gene | 5′-CTGTTGCTTATGGTGCTGCTG-3′ | 307 | 29 |
| CPHSP2672 | 5′-CCTCTTGGTGCTGGTGGAATA-3′ | ||||
| Probe 1 | GGP | GGL-GGR PCR product | 5′-TCGAGGACGTCGTCTCGAAGATCCAG-3′ | 23, 24 | |
| Probe 2 | CPHSP2475 | CPHSP2386-CPHSP2672 PCR product | 5′-CCATTATCACTCGGTTTAGA-3′ | 29 | |
Page 1746, Fig. 2: in panel C, “307 bp” should read “361 bp.”
Column 2, lines 37 and 38: “CPHSP2423 and CPHSP2764” should read “CPHSP2386 and CPHSP2672.”