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. 1984 Jul;75(3):781–787. doi: 10.1104/pp.75.3.781

Synthesis and Turnover of the Chloroplast Coupling Factor 1 in Chlamydomonas reinhardi1

Sabeeha Merchant 1, Bruce R Selman 1
PMCID: PMC1066993  PMID: 16663704

Abstract

Studies on the biosynthesis of the chloroplast coupling factor 1 (CF1) in Chlamydomonas reinhardi have been initiated. The ratio of CF1 to chlorophyll in the cell was shown to be independent of the density of the culture. No turnover of assembled CF1 could be detected, thus suggesting that CF1 was synthesized at a rate equivalent to that of net chlorophyll synthesis. A lag of between 5 to 7 minutes in the incorporation of radioactive precursor sulfate into assembled CF1 was measureable. This puts an upper limit on the pool size of any precursor to the assembled CF1 complex. The pool size is estimated to be equivalent to 1% of the total CF1 in the cell.

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Selected References

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