Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Jul;75(3):781–787. doi: 10.1104/pp.75.3.781

Synthesis and Turnover of the Chloroplast Coupling Factor 1 in Chlamydomonas reinhardi1

Sabeeha Merchant 1, Bruce R Selman 1
PMCID: PMC1066993  PMID: 16663704

Abstract

Studies on the biosynthesis of the chloroplast coupling factor 1 (CF1) in Chlamydomonas reinhardi have been initiated. The ratio of CF1 to chlorophyll in the cell was shown to be independent of the density of the culture. No turnover of assembled CF1 could be detected, thus suggesting that CF1 was synthesized at a rate equivalent to that of net chlorophyll synthesis. A lag of between 5 to 7 minutes in the incorporation of radioactive precursor sulfate into assembled CF1 was measureable. This puts an upper limit on the pool size of any precursor to the assembled CF1 complex. The pool size is estimated to be equivalent to 1% of the total CF1 in the cell.

Full text

PDF
781

Images in this article

782Fig. 1
on p.782
783Fig. 2
on p.783
785Fig. 6
on p.785

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Chua N. H., Schmidt G. W. Transport of proteins into mitochondria and chloroplasts. J Cell Biol. 1979 Jun;81(3):461–483. doi: 10.1083/jcb.81.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  6. De Heij J. T., Groot G. S. Spirodela oligorhiza chloroplast DNA codes for ATPase subunits alpha and beta. Immunological evidence from a coupled transcription-translation system. FEBS Lett. 1981 Nov 2;134(1):6–10. doi: 10.1016/0014-5793(81)80538-8. [DOI] [PubMed] [Google Scholar]
  7. Deno H., Shinozaki K., Sugiura M. Nucleotide sequence of tobacco chloroplast gene for the alpha subunit of proton-translocating ATPase. Nucleic Acids Res. 1983 Apr 11;11(7):2185–2191. doi: 10.1093/nar/11.7.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoffman-Falk H., Mattoo A. K., Marder J. B., Edelman M., Ellis R. J. General occurrence and structural similarity of the rapidly synthesized, 32,000-dalton protein of the chloroplast membrane. J Biol Chem. 1982 Apr 25;257(8):4583–4587. [PubMed] [Google Scholar]
  10. Howe C. J., Auffret A. D., Doherty A., Bowman C. M., Dyer T. A., Gray J. C. Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6903–6907. doi: 10.1073/pnas.79.22.6903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hurn B. A., Chantler S. M. Production of reagent antibodies. Methods Enzymol. 1980;70(A):104–142. doi: 10.1016/s0076-6879(80)70044-7. [DOI] [PubMed] [Google Scholar]
  12. Krebbers E. T., Larrinua I. M., McIntosh L., Bogorad L. The maize chloroplast genes for the beta and epsilon subunits of the photosynthetic coupling factor CF1 are fused. Nucleic Acids Res. 1982 Aug 25;10(16):4985–5002. doi: 10.1093/nar/10.16.4985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kwanyuen P., Wildman S. G. Evidence that genetic information for chloroplast coupling factor I is shared by nuclear and chloroplast DNA. Biochim Biophys Acta. 1978 May 10;502(2):269–275. doi: 10.1016/0005-2728(78)90048-8. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Mendiola-Morgenthaler L. R., Morgenthaler J. J., Price C. A. Synthesis of coupling factor CF1 protein by isolated spinach chloroplasts. FEBS Lett. 1976 Feb 1;62(1):96–100. doi: 10.1016/0014-5793(76)80025-7. [DOI] [PubMed] [Google Scholar]
  16. Nelson N., Nelson H., Schatz G. Biosynthesis and assembly of the proton-translocating adenosine triphosphatase complex from chloroplasts. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1361–1364. doi: 10.1073/pnas.77.3.1361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmidt G. W., Mishkind M. L. Rapid degradation of unassembled ribulose 1,5-bisphosphate carboxylase small subunits in chloroplasts. Proc Natl Acad Sci U S A. 1983 May;80(9):2632–2636. doi: 10.1073/pnas.80.9.2632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Selman-Reimer S., Merchant S., Selman B. R. Isolation, purification, and characterization of coupling factor 1 from Chlamydomonas reinhardi. Biochemistry. 1981 Sep 15;20(19):5476–5482. doi: 10.1021/bi00522a020. [DOI] [PubMed] [Google Scholar]
  19. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES