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. 1984 Jul;75(3):862–864. doi: 10.1104/pp.75.3.862

Measurement of the Pyrophosphate Content of Plant Tissues 1

Douglas A Smyth 1, Clanton C Black Jr 1
PMCID: PMC1067007  PMID: 16663718

Abstract

Pyrophosphate (PPi) was measured in pea (Pisum sativum L.) and corn (Zea mays L.) tissues by using an enzymic method based on PPi-dependent phosphofructokinase (PPi-PFK). Different organs of pea and corn seedlings were extracted to determine if PPi is present in sufficient amounts to serve as a substrate for the PPi-PFK activity in these tissues. The amount of PPi is at least 14% to 70% that of the ATP content in shoots and roots of peas and corn; and, for various plant tissues, ranges from 5 to 39 nanomoles of PPi per gram fresh tissue weight. We conclude that PPi is available as a substrate for the glycolytic function of PPi-PFK in plants. Furthermore, the presence of substrate amounts of PPi in plant tissues implies that plant energetics also must be evaluated in terms of PPi as an energy source and phosphate donor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carnal N. W., Black C. C. Phosphofructokinase activities in photosynthetic organisms : the occurrence of pyrophosphate-dependent 6-phosphofructokinase in plants and algae. Plant Physiol. 1983 Jan;71(1):150–155. doi: 10.1104/pp.71.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carnal N. W., Black C. C. Pyrophosphate-dependent 6-phosphofructokinase, a new glycolytic enzyme in pineapple leaves. Biochem Biophys Res Commun. 1979 Jan 15;86(1):20–26. doi: 10.1016/0006-291x(79)90376-0. [DOI] [PubMed] [Google Scholar]
  3. Heinonen J. K., Honkasalo S. H., Kukko E. I. A method for the concentration and for the colorimetric determination of nanomoles of inorganic pyrophosphate. Anal Biochem. 1981 Nov 1;117(2):293–300. doi: 10.1016/0003-2697(81)90725-9. [DOI] [PubMed] [Google Scholar]
  4. Huber S. C., Bickett D. M. Evidence for control of carbon partitioning by fructose 2,6-bisphosphate in spinach leaves. Plant Physiol. 1984 Feb;74(2):445–447. doi: 10.1104/pp.74.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Stitt M., Gerhardt R., Kürzel B., Heldt H. W. A role for fructose 2,6-bisphosphate in the regulation of sucrose synthesis in spinach leaves. Plant Physiol. 1983 Aug;72(4):1139–1141. doi: 10.1104/pp.72.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. TAUSSKY H. H., SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]
  7. Van Schaftingen E., Lederer B., Bartrons R., Hers H. G. A kinetic study of pyrophosphate: fructose-6-phosphate phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate. Eur J Biochem. 1982 Dec;129(1):191–195. doi: 10.1111/j.1432-1033.1982.tb07039.x. [DOI] [PubMed] [Google Scholar]
  8. Wu M. X., Smyth D. A., Black C. C. Fructose 2,6-bisphosphate and the regulation of pyrophosphate-dependent phosphofructokinase activity in germinating pea seeds. Plant Physiol. 1983 Sep;73(1):188–191. doi: 10.1104/pp.73.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]

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