Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Jul;75(3):873–875. doi: 10.1104/pp.75.3.873

Sulfate Assimilation in C4 Plants

Intercellular and Intracellular Location of ATP Sulfurylase, Cysteine Synthase, and Cystathionine β-Lyase in Maize Leaves

James N Burnell 1
PMCID: PMC1067010  PMID: 16663721

Abstract

The activity of ATP sulfurylase, cysteine synthase, and cystathionine β-lyase was measured in crude leaf extracts, bundle sheath strands, and mesophyll and bundle sheath chloroplasts to determine the location of sulfate assimilation of C4 plant leaves. Almost all the ATP sulfurylase activity was located in the bundle sheath chloroplasts while cysteine synthase and cystathionine β-lyase activity was located, in different proportions, in both chloroplast types.

A new spectrophotometric assay for measuring ATP sulfurylase activity is also described.

Full text

PDF
873

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burnell J. N., Hatch M. D. Regulation of C4 photosynthesis: catalytic phosphorylation as a prerequisite for ADP-mediated inactivation of pyruvate,Pi dikinase. Biochem Biophys Res Commun. 1984 Jan 13;118(1):65–72. doi: 10.1016/0006-291x(84)91068-4. [DOI] [PubMed] [Google Scholar]
  3. Burnell J. N., Whatley F. R. Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase. Biochim Biophys Acta. 1977 Mar 15;481(1):246–265. doi: 10.1016/0005-2744(77)90157-7. [DOI] [PubMed] [Google Scholar]
  4. Chapman K. S., Hatch M. D. Regulation of C4 photosynthesis: mechanism of activation and inactivation of extracted pyruvate, inorganic phosphate dikinase in relation to dark/light regulation. Arch Biochem Biophys. 1981 Aug;210(1):82–89. doi: 10.1016/0003-9861(81)90166-1. [DOI] [PubMed] [Google Scholar]
  5. Fankhauser H., Brunold C., Erismann K. H. Subcellular localization of O-acetylserine sulfhydrylase in spinach leaves. Experientia. 1976 Dec 15;32(12):1494–1497. doi: 10.1007/BF01924412. [DOI] [PubMed] [Google Scholar]
  6. Gerwick B. C., Black C. C. Sulfur assimilation in c(4) plants: intercellular compartmentation of adenosine 5'-triphosphate sulfurylase in crabgrass leaves. Plant Physiol. 1979 Oct;64(4):590–593. doi: 10.1104/pp.64.4.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerwick B. C., Ku S. B., Black C. C. Initiation of sulfate activation: a variation in c4 photosynthesis plants. Science. 1980 Jul 25;209(4455):513–515. doi: 10.1126/science.209.4455.513. [DOI] [PubMed] [Google Scholar]
  8. Masada M., Fukushima K., Tamura G. Cysteine synthase from rape leaves. J Biochem. 1975 May;77(5):1107–1115. doi: 10.1093/oxfordjournals.jbchem.a130811. [DOI] [PubMed] [Google Scholar]
  9. Smith I. K. Studies of l-Cysteine Biosynthetic Enzymes in Phaseolus vulgaris L. Plant Physiol. 1972 Oct;50(4):477–479. doi: 10.1104/pp.50.4.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wallsgrove R. M., Lea P. J., Miflin B. J. Intracellular localization of aspartate kinase and the enzymes of threonine and methionine biosynthesis in green leaves. Plant Physiol. 1983 Apr;71(4):780–784. doi: 10.1104/pp.71.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES