Abstract
The callus of Nicotiana rustica cv Gansu yellow flower and N. tabacum cv willow leaf were cultured on ordinary subculture medium (M-1) and on regeneration medium (M-2), respectively. No differentiation was observed in Gansu yellow flower tobacco callus cultures grown on both M-1 and M-2 medium. The respiration of both cultures was partially resistant to cyanide and markedly inhibited by m-chlorobenzhydroxamic acid. The relative contributions of alternative and cytochrome pathway were 31% and 47% of the total respiration, respectively, in M-1 callus cultures. The relative O2 uptake of the two pathways was not changed significantly in M-2 callus cultures. In subcultured M-1 callus cultures of Willow leaf tobacco, the respiration mediated via alternative pathway was about 29 to 38% of the total respiration, and the cytochrome pathway still was the major respiratory pathway. In M-2 callus cultures in which differentiation occurred, the relative contribution of alternative pathway increased to 41 to 47% of the total respiration, and the cytochrome pathway decreased considerably. These results suggested that the change of respiratory electron transport pathway was probably related to the differentiation of tobacco callus cultures.
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- Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
- Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. II. Control of the alternate pathway. J Biol Chem. 1973 May 25;248(10):3446–3450. [PubMed] [Google Scholar]
- Brown D. C., Thorpe T. A. Adenosine Phosphate and Nicotinamide Adenine Dinucleotide Pool Sizes during Shoot Initiation in Tobacco Callus. Plant Physiol. 1980 Apr;65(4):587–590. doi: 10.1104/pp.65.4.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hackett D. P., Haas D. W., Griffiths S. K., Niederpruem D. J. Studies on Development of Cyanide-resistant Respiration in Potato Tuber Slices. Plant Physiol. 1960 Jan;35(1):8–19. doi: 10.1104/pp.35.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Solomos T., Laties G. G. Similarities between the Actions of Ethylene and Cyanide in Initiating the Climacteric and Ripening of Avocados. Plant Physiol. 1974 Oct;54(4):506–511. doi: 10.1104/pp.54.4.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Theologis A., Laties G. G. Relative Contribution of Cytochrome-mediated and Cyanide-resistant Electron Transport in Fresh and Aged Potato Slices. Plant Physiol. 1978 Aug;62(2):232–237. doi: 10.1104/pp.62.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yentur S., Leopold A. C. Respiratory Transition during Seed Germination. Plant Physiol. 1976 Feb;57(2):274–276. doi: 10.1104/pp.57.2.274. [DOI] [PMC free article] [PubMed] [Google Scholar]