Abstract
A sensitive, specific enzyme immunoassay (EIA) for trans-zeatin riboside (ZR) and trans-zeatin (Z) in the 0.3 to 30 picomole range has been described. The reliability of the method for measuring ZR + Z in partially purified extracts of Zea mays L. tissues was verified by highperformance liquid chromatography. EIA measurements showed that there was a concentration gradient of ZR + Z along the length of the Zea stem. The topmost internodes, internodes 7 and 8 counting from the coleoptilar node, had the highest concentration (≃130 picomoles per gram fresh weight). Moving basipetally, the concentration dropped ≃10-fold to a minimum at internode 4, and then increased slightly in internodes 2 and 3. There were also gradients within each internode. The five lowest internodes contained the highest concentrations toward their apical end, the region which included the node; this asymmetry was less pronounced near the top of the plant.
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Selected References
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- Chen C. M., Petschow B. Cytokinin biosynthesis in cultured rootless tobacco plants. Plant Physiol. 1978 Dec;62(6):861–865. doi: 10.1104/pp.62.6.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ERLANGER B. F., BEISER S. M. ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA. Proc Natl Acad Sci U S A. 1964 Jul;52:68–74. doi: 10.1073/pnas.52.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goding J. W. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215–226. doi: 10.1016/0022-1759(76)90068-5. [DOI] [PubMed] [Google Scholar]
- Miller C. O. Evidence for the natural occurrence of zeatin and derivatives: compounds from maize which promote cell division. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1052–1058. doi: 10.1073/pnas.54.4.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodbard D., Lewald J. E. Computer analysis of radioligand assay and radioimmunoassay data. Acta Endocrinol Suppl (Copenh) 1970;147:79–103. doi: 10.1530/acta.0.065s079. [DOI] [PubMed] [Google Scholar]
- Rodbard D. Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays. Clin Chem. 1974 Oct;20(10):1255–1270. [PubMed] [Google Scholar]