Abstract
The initial products of photosynthesis by the C3 species Flaveria cronquistii, the C4 species F. trinervia, and the C3-C4 intermediate species F. ramosissima were determined using a pulse-chase technique with 14CO2-12CO2. The intermediate species F. ramosissima incorporated at least 42% of the total soluble 14C fixed into malate and aspartate after 10 seconds of photosynthesis in 14CO2, as compared with 90% for the C4 species F. trinervia and 5% for the C3 species F. cronquistii. In both F. ramosissima and F. trinervia, turnover of labeled malate and aspartate occurred during a chase period in 12CO2, although the rate of turnover was slower in the intermediate species. Relative to F. cronquistii, F. ramosissima showed a reduced incorporation of radioactivity into serine and glycine during the pulse period. These results indicate that a functional C4 pathway of photosynthesis is operating in F. ramosissima which can account for its reduced level of photorespiration, and that this species is a true biochemical intermediate between C3 and C4 plants.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chen T. M., Brown R. H., Black C. C. Photosynthetic CO(2) Fixation Products and Activities of Enzymes Related to Photosynthesis in Bermudagrass and Other Plants. Plant Physiol. 1971 Feb;47(2):199–203. doi: 10.1104/pp.47.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatch M. D. The C 4 -pathway of photosynthesis. Evidence for an intermediate pool of carbon dioxide and the identity of the donor C 4 -dicarboxylic acid. Biochem J. 1971 Nov;125(2):425–432. doi: 10.1042/bj1250425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holaday A. S., Chollet R. Photosynthetic/Photorespiratory Carbon Metabolism in the C(3)-C(4) Intermediate Species, Moricandia arvensis and Panicum milioides. Plant Physiol. 1983 Nov;73(3):740–745. doi: 10.1104/pp.73.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ku M. S., Monson R. K., Littlejohn R. O., Nakamoto H., Fisher D. B., Edwards G. E. Photosynthetic Characteristics of C(3)-C(4) Intermediate Flaveria Species : I. Leaf Anatomy, Photosynthetic Responses to O(2) and CO(2), and Activities of Key Enzymes in the C(3) and C(4) Pathways. Plant Physiol. 1983 Apr;71(4):944–948. doi: 10.1104/pp.71.4.944. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan J. A., Brown R. H. Photosynthesis in Grass Species Differing in Carbon Dioxide Fixation Pathways: II. A Search for Species with Intermediate Gas Exchange and Anatomical Characteristics. Plant Physiol. 1979 Aug;64(2):257–262. doi: 10.1104/pp.64.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan J. A., Brown R. H. Photosynthesis in Grass Species Differing in Carbon Dioxide Fixation Pathways: III. OXYGEN RESPONSE AND ENZYME ACTIVITIES OF SPECIES IN THE LAXA GROUP OF PANICUM. Plant Physiol. 1980 Jan;65(1):156–159. doi: 10.1104/pp.65.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rathnam C. K., Chollet R. Photosynthetic carbon metabolism in Panicum milioides, a C3-C4 intermediate species: evidence for a limited C4 dicarboxylic acid pathway of photosynthesis. Biochim Biophys Acta. 1979 Dec 6;548(3):500–519. doi: 10.1016/0005-2728(79)90061-6. [DOI] [PubMed] [Google Scholar]
- Schürmann P. Separation of phosphate esters and algal extracts by thin-layer electrophoresis and chromatography. J Chromatogr. 1969 Feb 25;39(4):507–509. [PubMed] [Google Scholar]
- Winter K., Usuda H., Tsuzuki M., Schmitt M., Edwards G. E., Thomas R. J., Evert R. F. Influence of Nitrate and Ammonia on Photosynthetic Characteristics and Leaf Anatomy of Moricandia arvensis. Plant Physiol. 1982 Aug;70(2):616–625. doi: 10.1104/pp.70.2.616. [DOI] [PMC free article] [PubMed] [Google Scholar]