Abstract
The activities of several enzymes of the citric acid and poly-β-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hydroxybutyrate dehydrogenase, β-ketothiolase, and pyruvate dehydrogenase complex increased whereas acetoacetate-succinyl-CoA transferase and isocitrate dehydrogenase decreased. Malate dehydrogenase activity remained constant. The amount of available acetyl-CoA, based on pyruvate dehydrogenase activity, should be sufficient to support both metabolic cycles concurrently. The temporal relationship between nitrogenase activity and poly-β-hydroxybutyrate accumulation has been reexamined.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bergersen J. F., Turner G. L. Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules. Biochim Biophys Acta. 1967 Aug 29;141(3):507–515. doi: 10.1016/0304-4165(67)90179-1. [DOI] [PubMed] [Google Scholar]
- Bresters T. W., de Kok A., Veeger C. The pyruvate-dehydrogenase complex from Azotobacter vinelandii. 2. Regulation of the activity. Eur J Biochem. 1975 Nov 15;59(2):347–353. doi: 10.1111/j.1432-1033.1975.tb02461.x. [DOI] [PubMed] [Google Scholar]
- Emerich D. W., Ruiz-Argüeso T., Ching T. M., Evans H. J. Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids. J Bacteriol. 1979 Jan;137(1):153–160. doi: 10.1128/jb.137.1.153-160.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grimes H., Fottrell P. F. Enzymes involved in glutamate metabolism in legume root nodules. Nature. 1966 Oct 15;212(5059):295–296. doi: 10.1038/212295a0. [DOI] [PubMed] [Google Scholar]
- Karr D. B., Waters J. K., Emerich D. W. Analysis of Poly-beta-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection. Appl Environ Microbiol. 1983 Dec;46(6):1339–1344. doi: 10.1128/aem.46.6.1339-1344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klucas R. V., Evans H. J. An electron donor system for nitrogenase-dependent acetylene reduction by extracts of soybean nodules. Plant Physiol. 1968 Sep;43(9):1458–1460. doi: 10.1104/pp.43.9.1458. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klucas R. V. Studies on soybean nodule senescence. Plant Physiol. 1974 Oct;54(4):612–616. doi: 10.1104/pp.54.4.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurz W. G., LaRUE T. A. Citric acid cycle enzymes and nitrogenase in nodules of Pisum sativum. Can J Microbiol. 1977 Sep;23(9):1197–1200. doi: 10.1139/m77-180. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Martínez-De Drets G., Arias A. Enzymatic basis for differentiation of Rhizobium into fast- and slow-growing groups. J Bacteriol. 1972 Jan;109(1):467–470. doi: 10.1128/jb.109.1.467-470.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Randall D. D., Williams M., Rapp B. J. Phosphorylation-dephosphorylation of pyruvate dehydrogenase complex from pea leaf mitochondria. Arch Biochem Biophys. 1981 Apr 1;207(2):437–444. doi: 10.1016/0003-9861(81)90051-5. [DOI] [PubMed] [Google Scholar]
- Rubin P. M., Randall D. D. Purification and characterization of pyruvate dehydrogenase complex from borccoli floral buds. Arch Biochem Biophys. 1977 Jan 30;178(2):342–349. doi: 10.1016/0003-9861(77)90202-8. [DOI] [PubMed] [Google Scholar]
- Senior P. J., Dawes E. A. The regulation of poly-beta-hydroxybutyrate metabolism in Azotobacter beijerinckii. Biochem J. 1973 May;134(1):225–238. doi: 10.1042/bj1340225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stovall I., Cole M. Organic Acid Metabolism by Isolated Rhizobium japonicum Bacteroids. Plant Physiol. 1978 May;61(5):787–790. doi: 10.1104/pp.61.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong P. P., Evans H. J. Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity. Plant Physiol. 1971 Jun;47(6):750–755. doi: 10.1104/pp.47.6.750. [DOI] [PMC free article] [PubMed] [Google Scholar]