Abstract
Studies with purified chloroplasts of Lupinus polyphyllus LINDL. leaflets indicate that the first two enzymes of quinolizidine alkaloid biosynthesis, lysine decarboxylase and 17-oxosparteine synthase, are localized in the chloroplast stroma. Thus, both enzymes share the same subcellular compartment as the biosynthetic pathway of lysine, the precursor of quinolizidine alkaloids. The activity of diaminopimelate decarboxylase, the final enzyme in lysine biosynthesis, is about two to three orders of magnitude higher than that of the enzymes of alkaloid formation.
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- Hartmann T., Schoofs G., Wink M. A chloroplast-localized lysine decarboxylase of Lupinus polyphyllus: the first enzyme in the biosynthetic pathway of quinolizidine alkaloids. FEBS Lett. 1980 Jun 16;115(1):35–38. doi: 10.1016/0014-5793(80)80721-6. [DOI] [PubMed] [Google Scholar]
- Mazelis M., Miflin B. J., Pratt H. M. A chloroplast-localized diaminopimelate decarboxylase in higher plants. FEBS Lett. 1976 Apr 15;64(1):197–200. doi: 10.1016/0014-5793(76)80282-7. [DOI] [PubMed] [Google Scholar]
- Mourioux G., Douce R. Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium. Plant Physiol. 1981 Mar;67(3):470–473. doi: 10.1104/pp.67.3.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHUETTE H. R., BOHLMANN F., REUSCHE W. [The biosynthesis of sparteine]. Arch Pharm. 1961 Oct;294/66:610–615. doi: 10.1002/ardp.19612941003. [DOI] [PubMed] [Google Scholar]
- Wallsgrove R. M., Mazelis M. The enzymology of lysine biosynthesis in higher plants: complete localization of the regulatory enzyme dihydrodipicolinate synthase in the chloroplasts of spinach leaves. FEBS Lett. 1980 Jul 28;116(2):189–192. doi: 10.1016/0014-5793(80)80640-5. [DOI] [PubMed] [Google Scholar]
- Wink M., Hartmann T. Cadaverine--pyruvate transamination: the principal step of enzymatic quinolizidine alkaloid biosynthesis in Lupinus polyphyllus cell suspension cultures. FEBS Lett. 1979 May 15;101(2):343–346. doi: 10.1016/0014-5793(79)81040-6. [DOI] [PubMed] [Google Scholar]