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. 1982 Jul;70(1):232–237. doi: 10.1104/pp.70.1.232

Evidence that Envelope and Thylakoid Membranes from Pea Chloroplasts Lack Glycoproteins 1

Kenneth Keegstra 1, Kenneth Cline 1
PMCID: PMC1067118  PMID: 16662452

Abstract

Envelope and thylakoid membranes from pea (Pisum sativum var. Laxton's Progress No. 9) chloroplasts were analyzed for the presence of glycoproteins using two different approaches. First, the sugar composition of delipidated membrane polypeptides was measured directly using gas chromatographic analysis. The virtual absence of sugars suggests that plastid membranes lack glycoproteins. Second, membrane polypeptides separated by sodium dodecyl sulfate gel electrophoresis were tested for reactivity toward three different lectins: Concanavalin A, Ricinus communis agglutinin, and wheat germ agglutinin. In each case, there was no reactivity between any of the lectins and the plastid polypeptides. Microsomal membranes from pea tissues were used as a positive control. Glycoproteins were readily detectable in microsomal membranes using either of the two techniques. From these results it was concluded that pea chloroplast membranes do not contain glycosylated polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreu J. M., Warth R., Muñoz E. Glycoprotein nature of energy-transducing ATPases. Chemical characterization of glycopeptides isolated from bacterial and chloroplast coupling factors. FEBS Lett. 1978 Feb 1;86(1):1–5. doi: 10.1016/0014-5793(78)80084-2. [DOI] [PubMed] [Google Scholar]
  2. Apel K. The light-harvesting chlorophylla a/b.protein complex of the green alga Acetabularia mediterranea. Isolation and characterization of two subunits. Biochim Biophys Acta. 1977 Nov 17;462(2):390–402. doi: 10.1016/0005-2728(77)90137-2. [DOI] [PubMed] [Google Scholar]
  3. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burke D., Keegstra K. Carbohydrate structure of Sindbis virus glycoprotein E2 from virus grown in hamster and chicken cells. J Virol. 1979 Feb;29(2):546–554. doi: 10.1128/jvi.29.2.546-554.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burridge K. Direct identification of specific glycoproteins and antigens in sodium dodecyl sulfate gels. Methods Enzymol. 1978;50:54–64. doi: 10.1016/0076-6879(78)50007-4. [DOI] [PubMed] [Google Scholar]
  6. Cline K., Andrews J., Mersey B., Newcomb E. H., Keegstra K. Separation and characterization of inner and outer envelope membranes of pea chloroplasts. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3595–3599. doi: 10.1073/pnas.78.6.3595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krusius T., Finne J., Margolis R. U., Margolis R. K. Structural features of microsomal, synaptosomal, mitochondrial, and soluble glycoproteins of brain. Biochemistry. 1978 Sep 5;17(18):3849–3854. doi: 10.1021/bi00611a026. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lamport D. T., Katona L., Roerig S. Galactosylserine in extensin. Biochem J. 1973 May;133(1):125–132. doi: 10.1042/bj1330125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  11. Mellor R. B., Krusius T., Lord J. M. Analysis of glycoconjugate saccharides in organelles isolated from castor bean endosperm. Plant Physiol. 1980 Jun;65(6):1073–1075. doi: 10.1104/pp.65.6.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nalin C. M., Cross R. L., Lucas J. J., Kohlbrenner W. E. Lack of evidence for covalently-bound carbohydrates in energy-transducing ATPases from mitochondria, bacteria, and chloroplasts. FEBS Lett. 1979 Aug 15;104(2):209–214. doi: 10.1016/0014-5793(79)80816-9. [DOI] [PubMed] [Google Scholar]
  13. Racusen D. Distribution of Protein-bound Hexosamine in Chloroplasts. Plant Physiol. 1976 Jan;57(1):53–54. doi: 10.1104/pp.57.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]

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