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. 1982 Aug;70(2):357–360. doi: 10.1104/pp.70.2.357

Biological Properties of d-Amino Acid Conjugates of 2,4-D 1

Gayle H Davidonis 1,2, Robert H Hamilton 1,2, Remo P Vallejo 1,2, Robert Buly 1,2, Ralph O Mumma 1,2
PMCID: PMC1067149  PMID: 16662495

Abstract

Some d-amino acid (glutamic acid, valine, or leucine) conjugates of 2,4-dichlorophenoxyacetic acid (2,4-D) at 10−5 molar, stimulated elongation of Avena sativa L. var Mariner coleoptile sections and growth of soybean (Glycine max. L. var Amsoy) tissue as much as did the l-amino acid conjugates at 10−6 molar. The d-methionine conjugate did not stimulate growth of soybean root callus tissue but did stimulate Avena elongation. The d-aspartic acid conjugate did not stimulate elongation of Avena coleoptiles but did stimulate growth of root callus tissue.

Pretreatment of root callus tissue with 100 micromolar 2,4-D-(d) -or (l) glutamic acid for 18 hours prior to incubation for 6 hours in [1-14C]2,4-D resulted in a greater uptake of [1-14C]2,4-D than did pretreatment with 2,4-d. Qualitatively the metabolites were similar in all preincubation treatments. Preincubation with 2,4-D-(d)-glutamic acid also resulted in larger free [14C]2,4-D pool and a significant increase in water-soluble conjugates when compared with 2,4-D, or 2,4-D-(l)-glutamic acid preincubations.

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Selected References

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