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. 1982 Aug;70(2):410–413. doi: 10.1104/pp.70.2.410

Identification of the Leaf Vacuole as a Major Nitrate Storage Pool 1

Robert C Granstedt 1,2, Ray C Huffaker 1
PMCID: PMC1067160  PMID: 16662506

Abstract

Highly purified vacuoles were isolated from protoplasts derived from green barley (Hordeum vulgare var. Numar) leaves, in order to determine their role as a NO3− storage sink. α-Mannosidase and acid phosphatase activities were used as markers to identify vacuoles, α-mannosidase being the more suitable. Nitrate and α-mannosidase, which were released from vacuoles destroyed during lysis of protoplasts, moved at unequal rates in the density gradient used for vacuole isolation. Purified vacuoles retained less NO3− than α-mannosidase during a single washing. Empirically determined corrections were used to account for NO3− movement in estimating the percentage of total cellular nitrate found in the vacuole. Vacuoles from plants grown in the presence of NO3− contained 58% of the total cellular NO3− and therefore represent a major NO3− storage pool.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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