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. 1982 Aug;70(2):579–584. doi: 10.1104/pp.70.2.579

Distribution of Lectins in Tissues, Derived Callus, and Roots of Psophocarpus tetragonolobus (Winged Bean) 1

Toula Meimeth 1,2, Kim Tran Thanh Van 1,2, Jean-Luc Marcotte 1,2, Toan Hanh Trinn 1,2, Adrienne E Clarke 1,2
PMCID: PMC1067191  PMID: 16662537

Abstract

The distribution of lectin in parental tissues, roots formed de novo from parental stem tissue, and derived callus cells of Psophocarpus tetragonolobus has been measured by hemagglutinating activity and radioimmunoassay. The antisera used for the radioimmunoassay was raised in rabbits to lectin isolated from seeds by affinity chromatography using insolubilized hog gastric mucin. The distribution of lectin in buffer extracts of the tissues (or cells) and the extracellular medium favors the tissues for in vitro grown roots, regardless of the culture conditions used. The lectin content of the extracellular medium is more significant for callus, regardless of its conditions of culture. The lectin activity of extracts of in vitro grown roots was higher than that of mature roots from whole plants. Differences in relative levels of lectin activity measured by hemagglutination and by radioimmunoassay, and differences in saccharide inhibition of hemagglutination, suggest the presence of multiple lectins in extracts of different tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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